Origin and immunolesioning of cholinergic basal forebrain innervation of cat primary auditory cortex

Kamke, M.R., Brown, M. and Irvine, D. R. F. (2005) Origin and immunolesioning of cholinergic basal forebrain innervation of cat primary auditory cortex. Hearing Research, 206 1-2: 89-106. doi:10.1016/j.heares.2004.12.014


Author Kamke, M.R.
Brown, M.
Irvine, D. R. F.
Title Origin and immunolesioning of cholinergic basal forebrain innervation of cat primary auditory cortex
Journal name Hearing Research   Check publisher's open access policy
ISSN 0378-5955
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.heares.2004.12.014
Volume 206
Issue 1-2
Start page 89
End page 106
Total pages 18
Place of publication Amsterdam
Publisher Elsevier/North-Holland Biomedical Press
Language eng
Subject 110903 Central Nervous System
110906 Sensory Systems
Abstract Numerous studies have implicated the cholinergic basal forebrain (cBF) in the modulation of auditory cortical responses. This study aimed to accurately define the sources of cBF input to primary auditory cortex (AI) and to assess the efficacy of a cholinergic immunotoxin in cat. Three anaesthetized cats received multiple injections of horseradish-peroxidase conjugated wheatgerm-agglutin into physiologically identified AI. Following one to two days survival, tetramethylbenzidine histochemistry revealed the greatest number of retrogradely labeled cells in ipsilateral putamen, globus pallidus and internal capsule, and smaller numbers in more medial nuclei of the basal forebrain (BF). Concurrent choline acetyltransferase immunohistochemistry showed that almost 80% of the retrogradely labeled cells in BF were cholinergic, with the vast majority of these cells arising from the more lateral BF nuclei identified above. In the second part of the study, unilateral intraparenchymal injections of the cholinergic immunotoxin ME20.4-SAP were made into the putamen/globus pallidus nuclei of six cats. Immuno- and histochemistry revealed a massive reduction in the number of cholinergic cells in and around the targeted area, and a corresponding reduction in the density of cholinergic fibers in auditory cortex. These results are discussed in terms of their implications for investigations of the role of the cBF in cortical plasticity.
Keyword AI
Acetylcholine
Immunohistochemistry
Immunotoxin
Retrograde
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Human Movement and Nutrition Sciences Publications
 
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