A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus)

McHale, M., Broderick, D., Ovenden, J. R. and Lanyon, J. M. (2008) A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). Molecular Ecology Resources, 8 3: 669-670.


Author McHale, M.
Broderick, D.
Ovenden, J. R.
Lanyon, J. M.
Title A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus)
Formatted title A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus)
Journal name Molecular Ecology Resources  (ERA 2012 Listed)    (ERA 2010 Rank C)   Check publisher's open access policy
Publication date 2008-06
Sub-type Article
DOI 10.1111/j.1471-8286.2007.02041.x
Volume number 8
Issue number 3
ISSN 1755-098X
Start page 669
End page 670
Total pages 2
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing Ltd
Collection year 2009
Language eng
Subject C1
960808 Marine Flora, Fauna and Biodiversity
060411 Population, Ecological and Evolutionary Genetics
Abstract Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.
Formatted abstract Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.
Keyword dugong
manatee
molecular sexing
SRY
ZFX
ZFY
Q-Index Code C1
Q-Index Status Confirmed Code

 
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