Nitric oxide production by a human osteoblast cell line stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide

Sosroseno, W, Bird, PS and Seymour, GJ (2009) Nitric oxide production by a human osteoblast cell line stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide. Oral Microbiology and Immunology, 24 1: 50-55. doi:10.1111/j.1399-302X.2008.00475.x


Author Sosroseno, W
Bird, PS
Seymour, GJ
Title Nitric oxide production by a human osteoblast cell line stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide
Formatted title
Nitric oxide production by a human osteoblast cell line stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide
Journal name Oral Microbiology and Immunology   Check publisher's open access policy
ISSN 0902-0055
Publication date 2009-02
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1399-302X.2008.00475.x
Volume 24
Issue 1
Start page 50
End page 55
Total pages 6
Place of publication Denmark
Publisher Wiley Blackwell Munksgaard
Collection year 2009
Language eng
Subject C1
920402 Dental Health
110599 Dentistry not elsewhere classified
060599 Microbiology not elsewhere classified
Abstract Background/aim: Human osteoblasts induced by inflammatory stimuli express an inducible nitric oxide synthase (iNOS). The aim of the present study was to test the hypothesis that Aggregatibacter actinomycetemcomitans lipopolysaccharide stimulates the production of nitric oxide (NO) by a human osteoblast-like cell line (HOS cells). Methods: Cells were stimulated directly with A. actinomycetemcomitans lipopolysaccharide or pretreated with the following l-NIL (an iNOS inhibitor), anti-CD14, Toll-like receptor 2 (TLR2), or TLR4 antibody before stimulation with A. actinomycetemcomitans lipopolysaccharide. The role of the cyclic nucleotides was assessed by pretreating the cells with the following; ODQ (a guanylyl cyclase inhibitor); SQ22536 (an adenylyl cyclase inhibitor); db-cAMP (a cyclic adenosine monophosphate analog); br-cGMP (a cyclic guanosine monophosphate analog); forskolin (an adenylyl cyclase activator), IBMX [a non-specific phosphodiesterase (PDE) inhibitor], or KT5720 [a protein kinase A (PKA) inhibitor]. The cells were also preincubated with genistein [a protein tyrosine kinase (PTK) inhibitor], bisindolylmaleimide [a protein kinase C (PKC) inhibitor], BPB [a phospholipase A2 (PLA2) inhibitor], and NDGA (a lipoxygenase inhibitor). The iNOS activity and nitrite production in the cell cultures were determined spectrophotometrically. Results: The results showed that A. actinomycetemcomitans lipopolysaccharide stimulated both iNOS activity and nitrite production by HOS cells; this was reduced by l-NIL, anti-CD14, or anti-TLR4 antibody, SQ22536, KT5720, genistein, bisindolylmaleimde, BPB, and NDGA, but was enhanced by db-cAMP, IBMX, and forskolin. Conclusion: These results therefore suggest that A. actinomycetemcomitans lipopolysaccharide may induce the production of NO by HOS cells via a CD14-TLR4 molecule complex, a cAMP-PKA pathway, as well as by a PTK, PKC, PLA2, and lipoxygenase-dependent mechanism.
Keyword lipopolysaccharide
Nitric Oxide
Osteoblasts
Actinobacillus Actinomycetemcomitans
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published Online: Dec 12 2008 6:16AM, then in print Feb 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Dentistry Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 02 Mar 2009, 15:05:31 EST by Margot Dallinger on behalf of School of Dentistry