Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation

Dobano, Carlota, Widera, Georg, Rabussay, Dietmar and Doolan, Denise L. (2007) Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation. Vaccine, 25 36: 6635-6645. doi:10.1016/j.vaccine.2007.06.036


Author Dobano, Carlota
Widera, Georg
Rabussay, Dietmar
Doolan, Denise L.
Title Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-110X
0264-410X
Publication date 2007-09-04
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2007.06.036
Volume 25
Issue 36
Start page 6635
End page 6645
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Subject 110704 Cellular Immunology
Abstract We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced byDNAplasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 g of DNA via EP was equivalent to 50 g of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN- responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.
Keyword Malaria
DNA vaccine
Electroporation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 02 Mar 2009, 11:09:52 EST by Paul Rollo on behalf of School of Medicine