Transcriptional Responses of Saccharomyces Cerevisiae to Preferred and Nonpreferred Nitrogen Sources in Glucose-limited Chemostat Cultures

Boer, V. M., Tai, S. L., Vuralhan, Z., Arifin, Y., Walsh, M. C., Piper, M. D. W., de Winde, J. H., Pronk, J. T. and Daran, J. M. (2007) Transcriptional Responses of Saccharomyces Cerevisiae to Preferred and Nonpreferred Nitrogen Sources in Glucose-limited Chemostat Cultures. Fems Yeast Research, 7 4: 604-620. doi:10.1111/j.1567-1364.2007.00220.x


Author Boer, V. M.
Tai, S. L.
Vuralhan, Z.
Arifin, Y.
Walsh, M. C.
Piper, M. D. W.
de Winde, J. H.
Pronk, J. T.
Daran, J. M.
Title Transcriptional Responses of Saccharomyces Cerevisiae to Preferred and Nonpreferred Nitrogen Sources in Glucose-limited Chemostat Cultures
Journal name Fems Yeast Research   Check publisher's open access policy
ISSN 1567-1356
1567-1364
Publication date 2007
Sub-type Article (original research)
DOI 10.1111/j.1567-1364.2007.00220.x
Volume 7
Issue 4
Start page 604
End page 620
Total pages 17
Place of publication Amsterdam
Publisher Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies
Language eng
Subject 060104 Cell Metabolism
Abstract Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae grown with six different nitrogen sources were subjected to transcriptome analysis. The use of chemostats enabled an analysis of nitrogen-source-dependent transcriptional regulation at a fixed specific growth rate. A selection of preferred (ammonium and asparagine) and nonpreferred (leucine, phenylalanine, methionine and proline) nitrogen sources was investigated. For each nitrogen source, distinct sets of genes were induced or repressed relative to the other five nitrogen sources. In total, 131 such 'signature transcripts' were identified in this study. In addition to signature transcripts, genes were identified that showed a transcriptional coresponse to two or more of the six nitrogen sources. For example, 33 genes were transcriptionally upregulated in leucine-grown, phenylalanine-grown and methionine-grown cultures; this was partly attributed to the involvement of common enzymes in the dissimilation of these amino acids. In addition to specific transcriptional responses elicited by individual nitrogen sources, their impact on global regulatory mechanisms such as nitrogen catabolite repression (NCR) were monitored. NCR-sensitive gene expression in the chemostat cultures showed that ammonium and asparagine were 'rich' nitrogen sources. By this criterion, leucine, proline and methionine were 'poor' nitrogen sources, and phenylalanine showed an 'intermediate' NCR response.
Keyword Saccharomyces cerevisiae
chemostat
transcriptome
amino acid
nitrogen catabolite repression
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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