Microbial ecology of the equine hindgut during oligofructose-induced laminitis

Milinovich, Gabriel J., Burrell, Paul C., Pollitt, Christopher C., Klieve, Athol V., Blackall, Linda L., Ouwerkerk, Diane, Woodland, Erika, Trott, Darren J., Kowalchuk, G., Heidelberg, J. and Bailey, M. J. (2008) Microbial ecology of the equine hindgut during oligofructose-induced laminitis. ISME Journal, 2 11: 1089-1100. doi:10.1038/ismej.2008.67


Author Milinovich, Gabriel J.
Burrell, Paul C.
Pollitt, Christopher C.
Klieve, Athol V.
Blackall, Linda L.
Ouwerkerk, Diane
Woodland, Erika
Trott, Darren J.
Kowalchuk, G.
Heidelberg, J.
Bailey, M. J.
Title Microbial ecology of the equine hindgut during oligofructose-induced laminitis
Journal name ISME Journal   Check publisher's open access policy
ISSN 1751-7362
1751-7370
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1038/ismej.2008.67
Open Access Status
Volume 2
Issue 11
Start page 1089
End page 1100
Total pages 12
Place of publication London, England, U.K.
Publisher Nature Publishing Group
Collection year 2009
Language eng
Subject C1
970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
070707 Veterinary Microbiology (excl. Virology)
0707 Veterinary Sciences
Abstract Alimentary carbohydrate overload is a significant cause of laminitis in horses and is correlated with drastic shifts in the composition of hindgut microbiota. Equine hindgut streptococcal species (EHSS), predominantly Streptococcus lutetiensis, have been shown to be the most common microorganisms culturable from the equine caecum prior to the onset of laminitis. However, the inherent biases of culture-based methods are estimated to preclude up to 70% of the normal caecal microbiota. The objective of this study was to evaluate bacterial population shifts occurring in the equine caecum throughout the course of oligofructose-induced laminitis using several culture-independent techniques and to correlate these with caecal lactate, volatile fatty acid and degrees of polymerization 3–7 fructo-oligosaccharide concentrations. Our data conclusively show that of the total microbiota present in the equine hindgut, the EHSS S. lutetiensis is the predominant microorganism that proliferates prior to the onset of laminitis, utilizing oligofructose to produce large quantities of lactate. Population shifts in lactobacilli and Escherichia coli subpopulations occur secondarily to the EHSS population shifts, thus confirming that lactobacilli and coliforms have no role in laminitis. A large, curved, Gram-negative rod previously observed during the early phases of laminitis induction was most closely related to the Anaerovibrio genus and most likely represents a new, yet to be cultured, genus and species. Correlation of fluorescence in situ hybridization and quantitative real-time PCR results provide evidence supporting the hypothesis that laminitis is associated with the death en masse and rapid cell lysis of EHSS. If EHSS are lysed, liberated cellular components may initiate laminitis.
Keyword Laminitis
Oligofructose
Streptococci
Hindgut
Equine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Corrigendum: Vol 2/11 pg 1169

 
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Created: Thu, 26 Feb 2009, 15:20:46 EST by Mrs Christine Wheatland on behalf of School of Veterinary Science