Respirometry: Anhydrous drierite equilibrates with carbon dioxide and increases washout times

White, Craig R., Portugal, Steven J., Martin, Graham R. and Butler, Patrick J. (2006) Respirometry: Anhydrous drierite equilibrates with carbon dioxide and increases washout times. Physiological and Biochemical Zoology, 79 5: 977-980. doi:10.1086/505994

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Author White, Craig R.
Portugal, Steven J.
Martin, Graham R.
Butler, Patrick J.
Title Respirometry: Anhydrous drierite equilibrates with carbon dioxide and increases washout times
Journal name Physiological and Biochemical Zoology   Check publisher's open access policy
ISSN 1522-2152
1537-5293
0031-935X
Publication date 2006-09
Sub-type Article (original research)
DOI 10.1086/505994
Open Access Status File (Publisher version)
Volume 79
Issue 5
Start page 977
End page 980
Total pages 4
Editor James W. Hicks
Place of publication Chicago, IL, U.S.A.
Publisher University of Chicago Press
Language eng
Subject 0608 Zoology
Formatted abstract
Respirometry is a standard technique in comparative physiology laboratories, with measurements of rates of O2 consumption and CO2 production available for hundreds of species. A common recommendation when carrying out respirometry is that water vapor be removed from the air stream before entering the analyzer. Often, this is accomplished with the use of chemical desiccants, such as Drierite. However, here we show that Drierite has an affinity for CO2 when new and completely anhydrous, and therefore it has an adverse effect on the washout characteristics of this gas. Exposing the Drierite to room air reduces this CO2 affinity, and a 2‐min exposure at 20°C and 50% relative humidity is sufficient to reduce the 99% washout time by almost a factor of 4, at the cost of only a 5% reduction in water vapor capacity. When Drierite is exhausted and then recharged according to the manufacturer’s instructions, the CO2 affinity is further reduced, and washout times are less than 60% greater than when no desiccant is used.
© 2006 by The University of Chicago. All rights reserved.
Keyword Oxygen-consumption
Systems
Metabolism
Design
Vo2
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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