Comparative analysis of anti-mouse SRY antibodies

Bradford, S. T., Wilhelm, D. and Koopman, P. (2007) Comparative analysis of anti-mouse SRY antibodies. Sexual Development, 1 5: 305-310. doi:10.1159/000108933


Author Bradford, S. T.
Wilhelm, D.
Koopman, P.
Title Comparative analysis of anti-mouse SRY antibodies
Journal name Sexual Development   Check publisher's open access policy
ISSN 1661-5425
Publication date 2007-11
Year available 2008
Sub-type Article (original research)
DOI 10.1159/000108933
Volume 1
Issue 5
Start page 305
End page 310
Total pages 5
Place of publication Basel, Switzerland
Publisher S. Karger
Collection year 2009
Language eng
Subject 270205 Genetic Development (incl. Sex Determination)
780105 Biological sciences
C1
Abstract The Y chromosome gene SRY is the initiator of male sexual differentiation in mammals, but the molecular and cellular mechanisms operating downstream of SRY remain undefined. A deeper understanding of these issues relies on the ability to visualize SRY protein endogenously under a number of experimental conditions. Here we compare the specificity and effectiveness of several available antibodies to mouse SRY. Two antibodies cross-reacted with other SOX proteins in immunofluorescence analyses of transfected cells, and one of these two was unable to detect SRY on Western blots. A third antibody was both avid and specific, and was able to detect endogenous SRY in developing Sertoli cells in mouse genital ridges. Our findings underline the need to distinguish between useful and spurious reagents for biochemical and immunolocalization studies involving mouse SRY protein.
Keyword Gonad Development
Immunohistochemistry
Mouse
Sex Determination
SRY
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Excellence in Research Australia (ERA) - Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 24 Feb 2009, 16:39:22 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience