Ankyrin repeat domain-encoding genes in the wPip strain of Wolbachia from the Culex pipiens group

Walker, Thomas, Klasson, Lisa, Sebalhia, Mohammed, Sanders, Mandy J., Thompson, Nicholas R., Parkhill, Julian and Sinkins, Steven P. (2007) Ankyrin repeat domain-encoding genes in the wPip strain of Wolbachia from the Culex pipiens group. BMC Biology, 5 39-1-39-9. doi:10.1186/1741-7007-5-39


Author Walker, Thomas
Klasson, Lisa
Sebalhia, Mohammed
Sanders, Mandy J.
Thompson, Nicholas R.
Parkhill, Julian
Sinkins, Steven P.
Title Ankyrin repeat domain-encoding genes in the wPip strain of Wolbachia from the Culex pipiens group
Formatted title Ankyrin repeat domain-encoding genes in the wPip strain of Wolbachia from the Culex pipiens group
Journal name BMC Biology   Check publisher's open access policy
ISSN 1741-7007
Publication date 2007-09-20
Sub-type Article (original research)
DOI 10.1186/1741-7007-5-39
Volume 5
Start page 39-1
End page 39-9
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Subject 060408 Genomics
Formatted abstract Background Wolbachia are obligate endosymbiotic bacteria maternally transmitted through the egg cytoplasm that are responsible for several reproductive disorders in their insect hosts, such as cytoplasmic incompatibility (CI) in infected mosquitoes. Species in the Culex pipiens complex display an unusually high number of Wolbachia-induced crossing types, and based on present data, only the wPip strain is present.

Results The sequencing of the wPip strain of Wolbachia revealed the presence of 60 ankyrin repeat domain (ANK) encoding genes and expression studies of these genes were carried out in adult mosquitoes. One of these ANK genes, pk2, is shown to be part of an operon of three prophage-associated genes with sex-specific expression, and is present in two identical copies in the genome. Another homolog of pk2 is also present that is differentially expressed in different Cx. pipiens group strains. A further two ANK genes showed sex-specific regulation in wPip-infected Cx. pipiens group adults.

Conclusion
The high number, variability and differential expression of ANK genes in wPip suggest an important role in Wolbachia biology, and the gene family provides both markers and promising candidates for the study of reproductive manipulation.
Keyword Ankryrin
Primer
DNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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