Regulation of the endosomal SNARE protein syntaxin 7 by colony-stimulating factpr 1 in macrophages

Achuthan, Adrian, Masendycz, Paul, Lopez, Jamie A., Nguyen, Thao, James, David E., Sweet, Matthew J., Hamilton, John A. and Scholz, Glen M. (2008) Regulation of the endosomal SNARE protein syntaxin 7 by colony-stimulating factpr 1 in macrophages. Molecular and Cellular Biology, 28 20: 6149-6159. doi:10.1128/MCB.00220-08

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Author Achuthan, Adrian
Masendycz, Paul
Lopez, Jamie A.
Nguyen, Thao
James, David E.
Sweet, Matthew J.
Hamilton, John A.
Scholz, Glen M.
Title Regulation of the endosomal SNARE protein syntaxin 7 by colony-stimulating factpr 1 in macrophages
Journal name Molecular and Cellular Biology   Check publisher's open access policy
ISSN 0270-7306
Publication date 2008-08-18
Year available 2008
Sub-type Article (original research)
DOI 10.1128/MCB.00220-08
Open Access Status File (Publisher version)
Volume 28
Issue 20
Start page 6149
End page 6159
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2009
Language eng
Abstract Colony-stimulating factor 1 (CSF-1) is the main growth factor controlling the development of macrophages from myeloid progenitor cells. However, CSF-1 also regulates some of the key effector functions of macrophages (e.g., phagocytosis and cytokine secretion). The endosomal SNARE protein syntaxin 7 (Stx7) regulates vesicle trafficking events involved in phagocytosis and cytokine secretion. Therefore, we investigated the ability of CSF-1 to regulate Stx7. CSF-1 upregulated Stx7 expression in primary mouse macrophages; it also upregulated expression of its SNARE partners Vti1b and VAMP8 but not Stx8. Additionally, CSF-1 induced the rapid serine phosphorylation of Stx7 and enhanced its binding to Vti1b, Stx8, and VAMP8. Bioinformatics analysis and results from experiments with kinase inhibitors suggested the CSF-1-induced phosphorylation of Stx7 was mediated by protein kinase C and Akt in response to phosphatidylinositol 3-kinase activation. Based on mutagenesis studies, CSF-1 appeared to increase the binding of Stx7 to its SNARE partners by inducing the phosphorylation of serine residues in the Habc domain and/or "linker" region of Stx7. Thus, CSF-1 is a key regulator of Stx7 expression and function in macrophages. Furthermore, the effects of CSF-1 on Stx7 may provide a mechanism for the regulation of macrophage effector functions by CSF-1.
Keyword Macrophages
Syntaxin 7
Q-Index Code C1
Q-Index Status Confirmed Code

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Created: Thu, 19 Feb 2009, 15:16:38 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience