Genetic and genomic insights into the role of benzoate-catabolic pathway redundancy in Burkholderia xenovorans LB400

Denef, V. J., Klappenbach , J. A., Patrauchan, M. A., Florizone, C., Rodrigues , J. L. M., Tsoi, T. V., Verstraete, W., Eltis, L. D. and Tiedje, J. M. (2006) Genetic and genomic insights into the role of benzoate-catabolic pathway redundancy in Burkholderia xenovorans LB400. Applied and Environmental Microbiology, 72 1: 585-595. doi:10.1128/AEM.72.1.585-595.2006

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ164836_OA.pdf Full text (open access) application/pdf 499.27KB 0

Author Denef, V. J.
Klappenbach , J. A.
Patrauchan, M. A.
Florizone, C.
Rodrigues , J. L. M.
Tsoi, T. V.
Verstraete, W.
Eltis, L. D.
Tiedje, J. M.
Title Genetic and genomic insights into the role of benzoate-catabolic pathway redundancy in Burkholderia xenovorans LB400
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 1098-5336 : 0099-224
Publication date 2006-01
Sub-type Article (original research)
DOI 10.1128/AEM.72.1.585-595.2006
Open Access Status File (Publisher version)
Volume 72
Issue 1
Start page 585
End page 595
Total pages 11
Place of publication Washington
Publisher American Society for Microbiology
Language eng
Subject 0605 Microbiology
Abstract Transcriptomic and proteomic analyses of Burkholderia xenovorans LB400, a potent polychlorinated biphenyl (PCB) degrader, have implicated growth substrate- and phase-dependent expression of three benzoate-catabolizing pathways: a catechol ortho cleavage (ben-cat) pathway and two benzoyl-coenzyme A pathways, encoded by gene clusters on the large chromosome (boxC) and the megaplasmid (boxM). To elucidate the significance of this apparent redundancy, we constructed mutants with deletions of the ben-cat pathway (the {Delta}benABCD::kan mutant), the boxC pathway (the {Delta}boxABC::kan mutant), and both pathways (the {Delta}benABCD{Delta} boxABC::kan mutant). All three mutants oxidized benzoate in resting-cell assays. However, the {Delta}benABCD::kan and {Delta}benABCD {Delta}boxABC::kan mutants grew at reduced rates on benzoate and displayed increased lag phases. By contrast, growth on succinate, on 4-hydroxybenzoate, and on biphenyl was unaffected. Microarray and proteomic analyses revealed that cells of the {Delta}benABCD::kan mutant growing on benzoate expressed both box pathways. Overall, these results indicate that all three pathways catabolize benzoate. Deletion of benABCD abolished the ability of LB400 to grow using 3-chlorobenzoate. None of the benzoate pathways could degrade 2- or 4-chlorobenzoate, indicating that the pathway redundancy does not directly contribute to LB400's PCB-degrading capacities. Finally, an extensive sigmaE-regulated oxidative stress response not present in wild-type LB400 grown on benzoate was detected in these deletion mutants, supporting our earlier suggestion that the box pathways are preferentially active under reduced oxygen tension. Our data further substantiate the expansive network of tightly interconnected and complexly regulated aromatic degradation pathways in LB400
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 66 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 63 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 19 Feb 2009, 09:26:43 EST by Judy Dingwall on behalf of Advanced Water Management Centre