Dynamics of an Oligotrophic Bacterial Aquifer Community during Contact with a Groundwater Plume Contaminated with Benzene, Toluene, Ethylbenzene, and Xylenes: an In Situ Mesocosm Study{dagger}

Hendrickx, Barbara, Dejonghe, Winnie, Boënne, Wesley, Brennerova, Maria, Cernik, Miroslav, Lederer, Tomas, Bucheli-Witschel, Margarete, Bastiaens, Leen, Verstraete, Willy, Top, Eva M., Diels, Ludo and Springael, Dirk (2005) Dynamics of an Oligotrophic Bacterial Aquifer Community during Contact with a Groundwater Plume Contaminated with Benzene, Toluene, Ethylbenzene, and Xylenes: an In Situ Mesocosm Study{dagger}. Applied and Environmental Microbiology, 71 7: 3815-3825. doi:10.1128/AEM.71.7.3815-3825.2005

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Author Hendrickx, Barbara
Dejonghe, Winnie
Boënne, Wesley
Brennerova, Maria
Cernik, Miroslav
Lederer, Tomas
Bucheli-Witschel, Margarete
Bastiaens, Leen
Verstraete, Willy
Top, Eva M.
Diels, Ludo
Springael, Dirk
Title Dynamics of an Oligotrophic Bacterial Aquifer Community during Contact with a Groundwater Plume Contaminated with Benzene, Toluene, Ethylbenzene, and Xylenes: an In Situ Mesocosm Study{dagger}
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 1098-5336
0099-2240
Publication date 2005-07
Sub-type Article (original research)
DOI 10.1128/AEM.71.7.3815-3825.2005
Open Access Status File (Publisher version)
Volume 71
Issue 7
Start page 3815
End page 3825
Total pages 11
Place of publication x
Publisher Amercian Society for Microbiology
Language eng
Subject 0605 Microbiology
Abstract An in situ mesocosm system was designed to monitor the in situ dynamics of the microbial community in polluted aquifers. The mesocosm system consists of a permeable membrane pocket filled with aquifer material and placed within a polypropylene holder, which is inserted below groundwater level in a monitoring well. After a specific time period, the microcosm is recovered from the well and its bacterial community is analyzed. Using this system, we examined the effect of benzene, toluene, ethylbenzene, and xylene (BTEX) contamination on the response of an aquifer bacterial community by denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rRNA genes and PCR detection of BTEX degradation genes. Mesocosms were filled with nonsterile or sterile aquifer material derived from an uncontaminated area and positioned in a well located in either the uncontaminated area or a nearby contaminated area. In the contaminated area, the bacterial community in the microcosms rapidly evolved into a stable community identical to that in the adjacent aquifer but different from that in the uncontaminated area. At the contaminated location, bacteria with tmoA- and xylM/xylE1-like BTEX catabolic genotypes colonized the aquifer, while at the uncontaminated location only tmoA-like genotypes were detected. The communities in the mesocosms and in the aquifer adjacent to the wells in the contaminated area consisted mainly of Proteobacteria. At the uncontaminated location, Actinobacteria and Proteobacteria were found. Our results indicate that communities with long-term stability in their structures follow the contamination plume and rapidly colonize downstream areas upon contamination
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Supplemental material (read only) - http://aem.asm.org/content/71/7/3815/suppl/DC1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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Created: Wed, 18 Feb 2009, 11:14:49 EST by Judy Dingwall on behalf of Advanced Water Management Centre