H2S degradation is reflected by both the activity and composition of the microbial community in a compost biofilter

Sercu, Bram, Boon, Nico, Verstraete, Willy and Van Langenhove, Herman (2006) H2S degradation is reflected by both the activity and composition of the microbial community in a compost biofilter. Journal Applied Microbiology and Biotechnology, 72 5: 1090-1098. doi:10.1007/s00253-006-0382-x


Author Sercu, Bram
Boon, Nico
Verstraete, Willy
Van Langenhove, Herman
Title H2S degradation is reflected by both the activity and composition of the microbial community in a compost biofilter
Formatted title
H2S degradation is reflected by both the activity and composition of the microbial community in a compost biofilter
Journal name Journal Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
1432-0614
Publication date 2006-10
Sub-type Article (original research)
DOI 10.1007/s00253-006-0382-x
Volume 72
Issue 5
Start page 1090
End page 1098
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Subject 0605 Microbiology
090703 Environmental Technologies
Formatted abstract
In this study, 16S rRNA- and rDNA-based denaturing gradient gel electrophoresis (DGGE) were used to study the temporal and spatial evolution of the microbial communities in a compost biofilter removing H2S and in a control biofilter without H2S loading. During the first 81 days of the experiment, the H2S removal efficiencies always exceeded 93% at loading rates between 4.1 and 30 g m−3 h−1. Afterwards, the H2S removal efficiency decreased to values between 44 and 71%. RNA-based DGGE analysis showed that H2S loading to the biofilter increased the stability of the active microbial community but decreased the activity-based diversity and evenness. The most intense band in both the RNA- and DNA-based DGGE patterns of the H2S-degrading biofilter represented the sulfur oxidizing bacterium Thiobacillus thioparus. This suggested that T. thioparus constituted a major part of the bacterial community and was an important primary degrader in the H2S-degrading biofilter. The decreasing H2S removal efficiencies near the end of the experiment were not accompanied by a substantial change of the DGGE patterns. Therefore, the decreased H2S removal was probably not caused by a failing microbiology but rather by a decrease of the mass transfer of substrates after agglutination of the compost particles.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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Created: Wed, 18 Feb 2009, 10:23:46 EST by Judy Dingwall on behalf of Advanced Water Management Centre