A gene expression signature of confinement in peripheral blood of red wolves (Canis rufus)

Kennerly, Erin, Ballmann, Anne, Martin, Stanton, Wolfinger, Russ, Gregory, Simon, Stoskopf, Michael and Gibson, Greg (2008) A gene expression signature of confinement in peripheral blood of red wolves (Canis rufus). Molecular Ecology, 17 11: 2782-2791. doi:10.1111/j.1365-294X.2008.03775.x


Author Kennerly, Erin
Ballmann, Anne
Martin, Stanton
Wolfinger, Russ
Gregory, Simon
Stoskopf, Michael
Gibson, Greg
Title A gene expression signature of confinement in peripheral blood of red wolves (Canis rufus)
Formatted title A gene expression signature of confinement in peripheral blood of red wolves (Canis rufus)
Journal name Molecular Ecology   Check publisher's open access policy
ISSN 0962-1083
Publication date 2008-06
Sub-type Article (original research)
DOI 10.1111/j.1365-294X.2008.03775.x
Volume 17
Issue 11
Start page 2782
End page 2791
Total pages 10
Place of publication Oxford, England
Publisher Wiley-Blackwell Publishing Ltd.
Language eng
Subject 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
070705 Veterinary Immunology
Abstract The stresses that animals experience as a result of modification of their ecological circumstances induce physiological changes that leave a signature in profiles of gene expression. We illustrate this concept in a comparison of free range and confined North American red wolves (Canis rufus). Transcription profiling of peripheral blood samples from 13 red wolf individuals in the Alligator River region of North Carolina revealed a strong signal of differentiation. Four hundred eighty-two out of 2980 transcripts detected on Illumina HumanRef8 oligonucleotide bead arrays were found to differentiate free range and confined wolves at a false discovery rate of 12.8% and P < 0.05. Over-representation of genes in focal adhesion, insulin signalling, proteasomal, and tryptophan metabolism pathways suggests the activation of pro-inflammatory and stress responses in confined animals. Consequently, characterization of differential transcript abundance in an accessible tissue such as peripheral blood identifies biomarkers that could be useful in animal management practices and for evaluating the impact of habitat changes on population health, particularly as attention turns to the impact of climate change on physiology and in turn species distributions.
Keyword conservation genetics
heterologous microarray
Illumina
pro-inflammatory response
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published Online: 2 May 2008.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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