Composition and Distribution of Extracellular Polymeric Substances in Aerobic Flocs and Granular Sludge

McSwain, B. S., Irvine, R. L., Hausner, M. and Wilderer, P. A. (2005) Composition and Distribution of Extracellular Polymeric Substances in Aerobic Flocs and Granular Sludge. Applied and Environmental Microbiology, 71 2: 1051-1057. doi:10.1128/AEM.71.2.1051-1057.2005

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Author McSwain, B. S.
Irvine, R. L.
Hausner, M.
Wilderer, P. A.
Title Composition and Distribution of Extracellular Polymeric Substances in Aerobic Flocs and Granular Sludge
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 1098-5336
Publication date 2005
Sub-type Article (original research)
DOI 10.1128/AEM.71.2.1051-1057.2005
Open Access Status File (Publisher version)
Volume 71
Issue 2
Start page 1051
End page 1057
Total pages 7
Place of publication Washington
Publisher American Society for Microbiology
Language eng
Subject 0605 Microbiology
Abstract Extracellular polymeric substances (EPS) were quantified in flocculent and aerobic granular sludge developed in two sequencing batch reactors with the same shear force but different settling times. Several EPS extraction methods were compared to investigate how different methods affect EPS chemical characterization, and fluorescent stains were used to visualize EPS in intact samples and 20-µm cryosections. Reactor 1 (operated with a 10-min settle) enriched predominantly flocculent sludge with a sludge volume index (SVI) of 120 ± 12 ml g–1, and reactor 2 (2-min settle time) formed compact aerobic granules with an SVI of 50 ± 2 ml g–1. EPS extraction by using a cation-exchange resin showed that proteins were more dominant than polysaccharides in all samples, and the protein content was 50% more in granular EPS than flocculent EPS. NaOH and heat extraction produced a higher protein and polysaccharide content from cell lysis. In situ EPS staining of granules showed that cells and polysaccharides were localized to the outer edge of granules, whereas the center was comprised mostly of proteins. These observations confirm the chemical extraction data and indicate that granule formation and stability are dependent on a noncellular, protein core. The comparison of EPS methods explains how significant cell lysis and contamination by dead biomass leads to different and opposing conclusions.
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Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
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Advanced Water Management Centre Publications
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