Occurrence and community composition of fast-growing Mycobacterium in soils contaminated with polycyclic aromatic hydrocarbons

Leys, Natalie M., Ryngaert, Annemie, Bastiaens, Leen, Wattiau, Pierre, Top, Eva M., Verstraete, Willy and Springael, Dirk (2005) Occurrence and community composition of fast-growing Mycobacterium in soils contaminated with polycyclic aromatic hydrocarbons. Fems Microbiology Ecology, 51 3: 375-388. doi:10.1016/j.femsec.2004.09.015


Author Leys, Natalie M.
Ryngaert, Annemie
Bastiaens, Leen
Wattiau, Pierre
Top, Eva M.
Verstraete, Willy
Springael, Dirk
Title Occurrence and community composition of fast-growing Mycobacterium in soils contaminated with polycyclic aromatic hydrocarbons
Journal name Fems Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2005-02
Sub-type Article (original research)
DOI 10.1016/j.femsec.2004.09.015
Volume 51
Issue 3
Start page 375
End page 388
Total pages 14
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject 050303 Soil Biology
Abstract Fast-growing mycobacteria are considered essential members of the polycyclic aromatic hydrocarbons (PAH) degrading bacterial community in PAH-contaminated soils. To study the natural role and diversity of the Mycobacterium community in contaminated soils, a culture-independent fingerprinting method based on PCR combined with denaturing gradient gel electrophoresis (DGGE) was developed. New PCR primers were selected which specifically targeted the 16S rRNA genes of fast-growing mycobacteria, and single-band DGGE profiles of amplicons were obtained for most Mycobacterium strains tested. Strains belonging to the same species revealed identical DGGE fingerprints, and in most cases, but not all, these fingerprints were typical for one species, allowing partial differentiation between species in a Mycobacterium community. Mycobacterium strains inoculated in soil were detected with a detection limit of 106 CFU g−1 of soil using the new primer set as such, or approximately 102 CFU g−1 in a nested PCR approach combining eubacterial and the Mycobacterium specific primers. Using the PCR-DGGE method, different species could be individually recognized in a mixed Mycobacterium community. This approach was used to rapidly assess the Mycobacterium community structure of several PAH-contaminated soils of diverse origin with different overall contamination profiles, pollution concentrations and chemical-physical soil characteristics. In the non-contaminated soil, most of the recovered 16SrRNA gene sequence did not match with previous described PAH-degrading Mycobacterium strains. In most PAH-contaminated soils, mycobacteria were detected which
Keyword PAH biodegradation
Mycobacterium
16S rRNA gene
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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Created: Tue, 17 Feb 2009, 11:21:58 EST by Judy Dingwall on behalf of Advanced Water Management Centre