Hygienization by anaerobic digestion: Comparison between evaluation by cultivation and quantitative real-time PCR

Lebuhn, M., Effenberger, M., Garcés, G., Gronauer, A. and Wilderer, P. (2005) Hygienization by anaerobic digestion: Comparison between evaluation by cultivation and quantitative real-time PCR. Water Science and Technology, 52 1-2: 93-99.

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Name Description MIMEType Size Downloads
Author Lebuhn, M.
Effenberger, M.
Garcés, G.
Gronauer, A.
Wilderer, P.
Title Hygienization by anaerobic digestion: Comparison between evaluation by cultivation and quantitative real-time PCR
Journal name Water Science and Technology   Check publisher's open access policy
ISSN 0273-1223
1996-9732
Publication date 2005
Sub-type Article (original research)
Volume 52
Issue 1-2
Start page 93
End page 99
Total pages 7
Place of publication London, United Kingdom
Publisher IWA Publishing
Language eng
Subject 090409 Wastewater Treatment Processes
Abstract In order to assess hygienization by anaerobic digestion, a comparison between evaluation by cultivation and quantitative real-time PCR (qPCR) including optimized DNA extraction and quantification was carried out for samples from a full-scale fermenter cascade (F1, mesophilic; F2, thermophilic; F3, mesophilic). The system was highly effective in inactivating (pathogenic) viable microorganisms, except for spore-formers. Conventionally performed cultivation underestimated viable organisms particularly in F2 and F3 by a factor of at least 10 as shown by data from extended incubation times, probably due to the rise of sublethally injured (active but not cultivable) cells. Incubation should hence be extended adequately in incubation-based hygiene monitoring of stressed samples, in order to minimize contamination risks. Although results from qPCR and cultivation agreed for the equilibrated compartments, considerably higher qPCR values were obtained for the fermenters. The difference probably corresponded to DNA copies from decayed cells that had not yet been degraded by the residual microbial activity. An extrapolation from qPCR determination to the quantity of viable organisms is hence not justified for samples that had been exposed to lethal stress.
Keyword Active but not cutivable cells
Anaerobic digestion
Hygienization
Overestimation by qPCR
qPCR
Underestimation by cultivation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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