Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities

Mertens, Birgit, Boon, Nico and Verstraete, Willy (2005) Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities. Environmental microbiology, 7 5: 660-669. doi:10.1111/j.1462-2920.2005.00735.x


Author Mertens, Birgit
Boon, Nico
Verstraete, Willy
Title Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities
Journal name Environmental microbiology   Check publisher's open access policy
ISSN 1462-2912
1462-2920
Publication date 2005-05
Sub-type Article (original research)
DOI 10.1111/j.1462-2920.2005.00735.x
Volume 7
Issue 5
Start page 660
End page 669
Total pages 10
Place of publication Oxford
Publisher Blackwell Science
Language eng
Subject 050303 Soil Biology
Abstract In the past decades, large amounts of non-insecticidal hexachlorocyclohexane (HCH) isomers (α-, β-, δ- and ɛ-HCH) have been dumped as side-products of the insecticide γ-HCH (lindane). This study investigates the effect of HCH isomers on methane oxidation, an important soil function performed by methanotrophic bacteria. Both activity and structure of the methanotrophic community were assessed, using methane oxidation assays and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) respectively. Methane oxidation assays with historically polluted soils revealed that on the long-term methane oxidation was inhibited by HCH pollution. PCR-DGGE and diversity analysis based on Lorenz curves showed that the type I methanotrophic community was less evenly distributed in historically HCH-polluted soils compared with less polluted reference soils. Short-term experiments with methane-enriched consortia further demonstrated that only γ- and δ-isomers inhibited methane oxidation. Type I methanotrophs of methane-enriched microbial consortia that received γ- or δ-HCH evolved towards higher species richness. Apparently, for historically HCH-polluted soils, a narrow community remained after long-term exposure while in case of short-term exposures, methane-enriched consortia were converted into less active, but richer communities when they were stressed by the presence of γ- or δ-HCH. This work demonstrates the importance of incorporating all isomers and possible other side-products in risk assessment studies of persistent organic pollutants and the use of structural analysis of type I methanotrophic communities as evaluating tool.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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Created: Mon, 16 Feb 2009, 17:38:25 EST by Judy Dingwall on behalf of Advanced Water Management Centre