Human colon microbiota transform polycyclic aromatic hydrocarbons to estrogenic metabolites

Van de Wiele, Tom, Vanhaecke, Lynn, Boeckaert, Charlotte, Peru, Kerry, Headley, John and Verstraete, Willy (2005) Human colon microbiota transform polycyclic aromatic hydrocarbons to estrogenic metabolites. Environmental Health Perspectives, 113 1: 6-10. doi:10.1289/ehp.7259


Author Van de Wiele, Tom
Vanhaecke, Lynn
Boeckaert, Charlotte
Peru, Kerry
Headley, John
Verstraete, Willy
Title Human colon microbiota transform polycyclic aromatic hydrocarbons to estrogenic metabolites
Journal name Environmental Health Perspectives   Check publisher's open access policy
ISSN 0091-6765
1552-9924
Publication date 2005-01
Year available 2005
Sub-type Article (original research)
DOI 10.1289/ehp.7259
Open Access Status DOI
Volume 113
Issue 1
Start page 6
End page 10
Total pages 5
Place of publication Washington, DC, United States
Publisher National Institute of Environmental Health Sciences
Language eng
Subject 110801 Medical Bacteriology
Abstract Ingestion is an important exposure route for polycyclic aromatic hydrocarbons (PAHs) to enter the human body. Although the formation of hazardous PAH metabolites by human biotransformation enzymes is well documented, nothing is known about the PAH transformation potency of human intestinal microbiota. Using a gastrointestinal simulator, we show that human intestinal microbiota can also bioactivate PAHs, more in particular to estrogenic metabolites. PAH compounds are not estrogenic, and indeed, stomach and small intestine digestions of 62.5 nmol naphthalene, phenanthrene, pyrene, and benzo(a)pyrene showed no estrogenic effects in the human estrogen receptor bioassay. In contrast, colon digests of these PAH compounds displayed estrogenicity, equivalent to 0.31, 2.14, 2.70, and 1.48 nmol 17alpha-ethynylestradiol (EE2), respectively. Inactivating the colon microbiota eliminated these estrogenic effects. Liquid chromatography-mass spectrometry analysis confirmed the microbial PAH transformation by the detection of PAH metabolites 1-hydroxypyrene and 7-hydroxybenzo(a)pyrene in colon digests of pyrene and benzo(a)pyrene. Furthermore, we show that colon digests of a PAH-contaminated soil (simulated ingestion dose of 5 g/day) displayed estrogenic activity equivalent to 0.58 nmol EE2, whereas stomach or small intestine digests did not. Although the matrix in which PAHs are ingested may result in lower exposure concentrations in the gut, our results imply that the PAH bioactivation potency of colon microbiota is not eliminated by the presence of soil. Moreover, because PAH toxicity is also linked to estrogenicity of the compounds, the PA
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
ERA 2012 Admin Only
Advanced Water Management Centre Publications
 
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Created: Mon, 16 Feb 2009, 13:51:55 EST by Judy Dingwall on behalf of Advanced Water Management Centre