A novel reductive dehalogenase, identified in a contaminated groundwater enrichment culture and in Desulfitobacterium dichloroeliminans strain DCA1, is linked to dehalogenation of 1,2-dichloroethane

Marzorati, Massimo, De Ferra, Francesca, Van Raemdonck, Hilde, Borin, Sara, Allifranchini, Elena, Carpani, Giovanna, Serbolisca, Luca, Verstraete, William, Boon, Nico and Daffonchio, Daniele (2007) A novel reductive dehalogenase, identified in a contaminated groundwater enrichment culture and in Desulfitobacterium dichloroeliminans strain DCA1, is linked to dehalogenation of 1,2-dichloroethane. Applied and Environmental Microbiology, 73 9: 2990-2999. doi:10.1128/AEM.02748-06

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Author Marzorati, Massimo
De Ferra, Francesca
Van Raemdonck, Hilde
Borin, Sara
Allifranchini, Elena
Carpani, Giovanna
Serbolisca, Luca
Verstraete, William
Boon, Nico
Daffonchio, Daniele
Title A novel reductive dehalogenase, identified in a contaminated groundwater enrichment culture and in Desulfitobacterium dichloroeliminans strain DCA1, is linked to dehalogenation of 1,2-dichloroethane
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2007
Sub-type Article (original research)
DOI 10.1128/AEM.02748-06
Open Access Status File (Publisher version)
Volume 73
Issue 9
Start page 2990
End page 2999
Total pages 10
Place of publication Washington, D.C
Publisher American Society for Microbiology
Language eng
Subject 100203 Bioremediation
Abstract A mixed culture dechlorinating 1,2-dichloroethane (1,2-DCA) to ethene was enriched from groundwater that had been subjected to long-term contamination. In the metagenome of the enrichment, a 7-kb reductive dehalogenase (RD) gene cluster sequence was detected by inverse and direct PCR. The RD gene cluster had four open reading frames (ORF) showing 99% nucleotide identity with pceB, pceC, pceT, and orf1 of Dehalobacter restrictus strain DSMZ 9455T, a bacterium able to dechlorinate chlorinated ethenes. However, dcaA, the ORF encoding the catalytic subunit, showed only 94% nucleotide and 90% amino acid identity with pceA of strain DSMZ 9455T. Fifty-three percent of the amino acid differences were localized in two defined regions of the predicted protein. Exposure of the culture to 1,2-DCA and lactate increased the dcaA gene copy number by 2 log units, and under these conditions the dcaA and dcaB genes were actively transcribed. A very similar RD gene cluster with 98% identity in the dcaA gene sequence was identified in Desulfitobacterium dichloroeliminans strain DCA1, the only known isolate that selectively dechlorinates 1,2-DCA but not chlorinated ethenes. The dcaA gene of strain DCA1 possesses the same amino acid motifs as the new dcaA gene. Southern hybridization using total genomic DNA of strain DCA1 with dcaA gene-specific and dcaB- and pceB-targeting probes indicated the presence of two identical or highly similar dehalogenase gene clusters. In conclusion, these data suggest that the newly described RDs are specifically adapted to 1,2-DCA dechlorination.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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Created: Mon, 16 Feb 2009, 12:21:22 EST by Ms Sarada Rao on behalf of Advanced Water Management Centre