Strain-Specific Ureolytic Microbial Calcium Carbonate Precipitation

Hammes, Frederik, Boon, Nico, de Villiers, Johan, Verstraete, Willy and Douglas Siciliano, Steven (2003) Strain-Specific Ureolytic Microbial Calcium Carbonate Precipitation. Applied and Environmental Microbiology, 69 8: 4901-4909. doi:10.1128/AEM.69.8.4901-4909.2003

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Author Hammes, Frederik
Boon, Nico
de Villiers, Johan
Verstraete, Willy
Douglas Siciliano, Steven
Title Strain-Specific Ureolytic Microbial Calcium Carbonate Precipitation
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2003-08
Sub-type Article (original research)
DOI 10.1128/AEM.69.8.4901-4909.2003
Open Access Status File (Publisher version)
Volume 69
Issue 8
Start page 4901
End page 4909
Total pages 9
Place of publication Washington, D.C.
Publisher American Society for Microbiology
Language eng
Subject 0605 Microbiology
Abstract During a study of ureolytic microbial calcium carbonate (CaCO3) precipitation by bacterial isolates collected from different environmental samples, morphological differences were observed in the large CaCO3 crystal aggregates precipitated within bacterial colonies grown on agar. Based on these differences, 12 isolates were selected for further study. We hypothesized that the striking differences in crystal morphology were the result of different microbial species or, alternatively, differences in the functional attributes of the isolates selected. Sequencing of 16S rRNA genes showed that all of the isolates were phylogenetically closely related to the Bacillus sphaericus group. Urease gene diversity among the isolates was examined by using a novel application of PCR-denaturing gradient gel electrophoresis (DGGE). This approach revealed significant differences between the isolates. Moreover, for several isolates, multiple bands appeared on the DGGE gels, suggesting the apparent presence of different urease genes in these isolates. The substrate affinities (Km) and maximum hydrolysis rates (Vmax) of crude enzyme extracts differed considerably for the different strains. For certain isolates, the urease activity increased up to 10-fold in the presence of 30 mM calcium, and apparently this contributed to the characteristic crystal formation by these isolates. We show that strain-specific calcification occurred during ureolytic microbial carbonate precipitation. The specificity was mainly due to differences in urease expression and the response to calcium.
Keyword Microbiology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
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Created: Mon, 16 Feb 2009, 09:38:31 EST by Ms Karen Naughton on behalf of Advanced Water Management Centre