Analysis of Cell Movement in Amphimedon Embryos by Injection of Fluorescent Tracers

Adamska, Maja and Degnan, Bernard M. (2008) Analysis of Cell Movement in Amphimedon Embryos by Injection of Fluorescent Tracers. Cold Spring Harbor Protocols, 3 12: .


Author Adamska, Maja
Degnan, Bernard M.
Title Analysis of Cell Movement in Amphimedon Embryos by Injection of Fluorescent Tracers
Formatted title Analysis of Cell Movement in Amphimedon Embryos by Injection of Fluorescent Tracers
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1559-6095
Publication date 2008-12
Sub-type Article (original research)
DOI 10.1101/pdb.prot5097
Volume 3
Issue 12
Total pages 4
Place of publication United States
Publisher Cold Spring Harbor Laboratory Press
Collection year 2009
Language eng
Subject C1
960808 Marine Flora, Fauna and Biodiversity
060305 Evolution of Developmental Systems
Formatted abstract Although attempts to culture prepigmentation-stage embryos (i.e., blastulas and early gastrulas) outside of brood chambers have so far been unsuccessful in Amphimedon, it is possible to manipulate embryos within the brood chamber and follow their development under laboratory conditions. This protocol describes microinjection of lipophilic tracers such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) into embryos embedded in their native brood chamber. DiI does not appear to perturb embryonic development and is relatively resistant to photobleaching. As long as care is taken not to damage the fragile embryos during observation and photography, the same embryo can be photographed multiple times, permitting its development to be tracked (up to 4 wk) from early cleavage stages to hatching of free-swimming parenchymella larvae. The embryos or larvae also can be fixed without loss of fluorescence. This method also can be used to deliver other types of solutions to embryos or individual cells of early embryos.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biological Sciences Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 57 Abstract Views  -  Detailed Statistics
Created: Fri, 13 Feb 2009, 18:31:19 EST by Gail Walter on behalf of School of Biological Sciences