Performance and microbial analysis of defined and non-defined inocula for the removal of dimethyl sulfide in a biotrickling filter

Sercu, B., Boon, N., Vander Beken, S., Verstraete, W. and Van Langenhove, H. (2007) Performance and microbial analysis of defined and non-defined inocula for the removal of dimethyl sulfide in a biotrickling filter. Biotechnology and Bioengineering, 96 4: 661-672. doi:10.1002/bit.21059


Author Sercu, B.
Boon, N.
Vander Beken, S.
Verstraete, W.
Van Langenhove, H.
Title Performance and microbial analysis of defined and non-defined inocula for the removal of dimethyl sulfide in a biotrickling filter
Journal name Biotechnology and Bioengineering   Check publisher's open access policy
ISSN 0006-3592
1097-0290
Publication date 2007-03
Sub-type Article (original research)
DOI 10.1002/bit.21059
Volume 96
Issue 4
Start page 661
End page 672
Total pages 12
Place of publication New York
Publisher Wiley Interscience
Language eng
Subject 090703 Environmental Technologies
Abstract The performance and microbial communities of three differently inoculated biotrickling filters removing dimethyl sulfide (DMS) were compared. The biotrickling filters were inoculated with Thiobacillus thioparus TK-m (THIO), sludge (HANDS) and sludge + T. thioparus TK-m + Hyphomicrobium VS (HANDS++), respectively. The criteria investigated were length of the start-up period, the maximum elimination capacity, and the effects of intermittent loading rates, low pH, peak loading and very low loading rate on the DMS removal efficiency. The HANDS++ reactor exhibited the best performance considering all treatments. HANDS performed almost equally well as HANDS++, except during the determination of the ECmax, while THIO was generally the least efficient. During stable DMS loading at concentrations of 20 ppmv or lower, all reactors exhibited similar and high removal efficiencies (>99%). Denaturing gradient gel electrophoresis (DGGE) analysis showed the establishment of T. thioparus in the biofilm of all reactors, but not of Hyphomicrobium VS. Quantitative monitoring of the introduced bacterial strains was performed with a newly developed real-time PCR protocol. Initially, the inoculated strains were exclusively found in the reactors in which they were added. Afterwards, however, both strains developed in the biofilm of all three reactors, although T. thioparus attained higher cell densities than Hyphomicrobium. The presence of T. thioparus in THIO was related with the DMS loading rates that were applied, in the sense that intermittent DMS loading and very low DMS loading rates (0.5 ppmv) induced a decrease in gene copy numbers. Real-time PCR and DGGE both gave consistent results regarding the presence of Hyphomicrobium VS and Thiobacillus thioparus TK-m in the reactors. Only real-time PCR could be used to detect bacteria comprising of less than 1.4% of the total bacterial community (105 copies ring-1).
Keyword Biotrickling filter
Denaturing gradient gel electrophoresis
Dimethyl sulfide
Inoculum selection
Real-time PCR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 22 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 13 Feb 2009, 17:19:38 EST by Paul Rollo on behalf of Advanced Water Management Centre