Poly-β-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii

Halet, Dirk, Defoirdt, Tom, Damme, Petra Van, Vervaeren, Han, Forrez, Ilse, Van de Wiele, Tom, Boon, Nico, Sorgeloos, Patrick, Bossier, Peter and Verstraete, Willy (2007) Poly-β-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii. FEMS Microbiology Ecology, 60 3: 363-369. doi:10.1111/j.1574-6941.2007.00305.x


Author Halet, Dirk
Defoirdt, Tom
Damme, Petra Van
Vervaeren, Han
Forrez, Ilse
Van de Wiele, Tom
Boon, Nico
Sorgeloos, Patrick
Bossier, Peter
Verstraete, Willy
Title Poly-β-hydroxybutyrate-accumulating bacteria protect gnotobiotic Artemia franciscana from pathogenic Vibrio campbellii
Journal name FEMS Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2007-03-28
Sub-type Article (original research)
DOI 10.1111/j.1574-6941.2007.00305.x
Volume 60
Issue 3
Start page 363
End page 369
Total pages 7
Place of publication Oxford, England
Publisher Blackwell
Language eng
Subject 0605 Microbiology
Abstract A poly-β-hydroxybutyrate (PHB)-accumulating enrichment culture was obtained using activated sludge from a polyphosphate-accumulating reactor as inoculum. PHB accumulated by the enrichment culture significantly enhanced the survival of Artemia nauplii, infected with the virulent pathogen Vibrio campbellii LMG 21363. A strain was isolated from the enrichment culture, based on its ability to accumulate PHB, and 16S rRNA gene sequencing of the isolate revealed 99% sequence similarity to Brachymonas denitrificans AS-P1. The isolate, named PHB2, showed good PHB-accumulating activity (up to 32% of the cell dry weight). PHB accumulated by isolate PHB2 was able to protect Artemia completely from the V. campbellii strain. Our data indicate that PHB-accumulating bacteria, such as B. denitrificans PHB2, could be used as an an effective and economically interesting alternative strategy to control infections in aquaculture.
Keyword brine shrimp
PHB
luminescent vibriosis
Brachymonas denitrificans
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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