Identification of human fecal pollution sources in a coastal area: a case study at Oostende (Belgium)

Seurinck, Sylvie, Verdievel, Martin, Verstraete, Willy and Siciliano, Steven D. (2006) Identification of human fecal pollution sources in a coastal area: a case study at Oostende (Belgium). Journal of Water and Health, 4 2: 167-175. doi:10.2166/wh.2006.069


Author Seurinck, Sylvie
Verdievel, Martin
Verstraete, Willy
Siciliano, Steven D.
Title Identification of human fecal pollution sources in a coastal area: a case study at Oostende (Belgium)
Journal name Journal of Water and Health   Check publisher's open access policy
ISSN 1477-8920
Publication date 2006
Sub-type Article (original research)
DOI 10.2166/wh.2006.069
Volume 4
Issue 2
Start page 167
End page 175
Total pages 9
Place of publication London; Avenel, NJ
Publisher IWA Publishing
Language eng
Subject 050206 Environmental Monitoring
Abstract From April to June 2001, a monitoring study at Oostende (Belgium) was conducted to obtain an insight into fecal pollution impairing water quality at this coastal area. Eight sampling sites were selected based on the historically low water quality at these sites compared to the remainder of the area. Indicator organisms such as fecal coliforms, Escherichia coli and fecal streptococci were monitored by plating. A real-time PCR assay for quantification of the human-specific HF183 Bacteroides 16S rRNA genetic marker was used to detect human fecal pollution at the sampling sites. Human fecal pollution was detected at all sampling sites. However, the frequency of detection ranged from 30-100% and the amount of human-specific Bacteroides markers recorded varied between the sampling sites. Concentrations of 10(7) human-specific Bacteroides markers per 1 to levels below the detection limit of the real-time PCR assay were recorded. Our results indicate that human fecal pollution is a re-occurring problem in certain areas. Of all the environmental parameters monitored during the study, only rainfall was strongly related to the detection of the indicator organisms and the human-specific Bacteroides marker.
Formatted abstract
From April to June 2001, a monitoring study at Oostende (Belgium) was conducted to obtain an insight into fecal pollution impairing water quality at this coastal area. Eight sampling sites were selected based on the historically low water quality at these sites compared to the remainder of the area. Indicator organisms such as fecal coliforms, Escherichia coli and fecal streptococci were monitored by plating. A real-time PCR assay for quantification of the human-specific HF183 Bacteroides 16S rRNA genetic marker was used to detect human fecal pollution at the sampling sites. Human fecal pollution was detected at all sampling sites. However, the frequency of detection ranged from 30-100% and the amount of human-specific Bacteroides markers recorded varied between the sampling sites. Concentrations of 10(7) human-specific Bacteroides markers per 1 to levels below the detection limit of the real-time PCR assay were recorded. Our results indicate that human fecal pollution is a re-occurring problem in certain areas. Of all the environmental parameters monitored during the study, only rainfall was strongly related to the detection of the indicator organisms and the human-specific Bacteroides marker.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
 
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Created: Fri, 13 Feb 2009, 13:48:02 EST by Joanne Mellor on behalf of Advanced Water Management Centre