Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-Degrading Variovorax Strain

Dejonghe, Winnie, Berteloot, Ellen, Goris, Johan, Boon, Nico, Crul, Katrien, Maertens, Siska, Monica Hofte, Monica, De Vos, Paul, Verstraete, Willy and Top, Eva M. (2003) Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-Degrading Variovorax Strain. Applied and Environmental Microbiology, 69 3: 1532-1541. doi:10.1128/AEM.69.3.1532-1541.2003

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Author Dejonghe, Winnie
Berteloot, Ellen
Goris, Johan
Boon, Nico
Crul, Katrien
Maertens, Siska
Monica Hofte, Monica
De Vos, Paul
Verstraete, Willy
Top, Eva M.
Title Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-Degrading Variovorax Strain
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2003-03
Sub-type Article (original research)
DOI 10.1128/AEM.69.3.1532-1541.2003
Open Access Status File (Publisher version)
Volume 69
Issue 3
Start page 1532
End page 1541
Total pages 10
Place of publication Washington, D.C.
Publisher American Society for Microbiology
Language eng
Subject 0605 Microbiology
Abstract The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and biodegradation assays. Three strains, Variovorax sp. strain WDL1, Delftia acidovorans WDL34, and Pseudomonas sp. strain WDL5, were isolated directly from the linuron-degrading culture. In addition, subculture of this enrichment culture on potential intermediates in the degradation pathway of linuron (i.e., N,O-dimethylhydroxylamine and 3-chloroaniline) resulted in the isolation of, respectively, Hyphomicrobium sulfonivorans WDL6 and Comamonas testosteroni WDL7. Of these five strains, only Variovorax sp. strain WDL1 was able to use linuron as the sole source of C, N, and energy. WDL1 first converted linuron to 3,4-dichloroaniline (3,4-DCA), which transiently accumulated in the medium but was subsequently degraded. To the best of our knowledge, this is the first report of a strain that degrades linuron further than the aromatic intermediates. Interestingly, the rate of linuron degradation by strain WDL1 was lower than that for the consortium, but was clearly increased when WDL1 was coinoculated with each of the other four strains. D. acidovorans WDL34 and C. testosteroni WDL7 were found to be responsible for degradation of the intermediate 3,4-DCA, and H. sulfonivorans WDL6 was the only strain able to degrade N,O-dimethylhydroxylamine. The role of Pseudomonas sp. strain WDL5 needs to be further elucidated. The degradation of linuron can thus be performed by a single isolate, Variovorax sp. strain WDL1, but is stimulated by a synergistic interaction with the other strains isolated from the same linuron-degrading culture.
Keyword Microbiology
linuron degradation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 140 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 13 Feb 2009, 13:05:54 EST by Ms Karen Naughton on behalf of Advanced Water Management Centre