The Aquatic Automated Dosing and Maintenance System (AADAMS)

Kline, David I., Bryant, John, Kisflaudy, Eddie, Rohwer, Gary, Nostropaur, Fernando, Grayson, Jodi, Knowlton, Nancy and Rohwer, Forest (2006) The Aquatic Automated Dosing and Maintenance System (AADAMS). Limnology and Oceanography: Methods, 4 6: 184-192. doi:10.4319/lom.2006.4.184

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Author Kline, David I.
Bryant, John
Kisflaudy, Eddie
Rohwer, Gary
Nostropaur, Fernando
Grayson, Jodi
Knowlton, Nancy
Rohwer, Forest
Title The Aquatic Automated Dosing and Maintenance System (AADAMS)
Journal name Limnology and Oceanography: Methods   Check publisher's open access policy
ISSN 1541-5856
Publication date 2006-06
Year available 2006
Sub-type Article (original research)
DOI 10.4319/lom.2006.4.184
Open Access Status File (Publisher version)
Volume 4
Issue 6
Start page 184
End page 192
Total pages 9
Place of publication Waco, TX, United States
Publisher American Society of Limnology and Oceanography
Language eng
Subject 060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)
Abstract The maintenance and dosing of aquatic organisms, such as corals and mollusks, are essential for ecotoxicology studies, yet it is difficult to maintain many of these sensitive organisms for an extended period. Consequently, many previous aquatic ecotoxicology experiments have been limited in their number of replicates and maintained in one or a few experimental aquaria, with only a limited number of stressors tested in each experiment. Here we describe a modular system that overcomes many of the difficulties of maintaining large numbers of sensitive aquatic organisms in separate containers, and allows testing of a large suite of stressors in each experiment. The AADAMS (aquatic automated dosing and maintenance system) allows testing of 40 independent stressors with 10 independent replicates per stressor (400 individuals total). The AADAMS provides surge and regular water changes simultaneously with accurate dosing via Venturi valves. In a series of experiments over a 1-year period, the AADAMS was used to test the effects of various factors affecting water quality on Caribbean coral reefs. Roofing tar and road asphalt were two of the most damaging pollutants tested, with LD50 values (lethal dose that killed 50% of the corals) of 0.013 g L–1 and 0.079 g L–1, respectively, thus suggesting that runoff from roads and near-shore construction could be contributing to reef decline. The AADAMS is an accurate, reliable system for highly replicated ecotoxicological studies of sensitive aquatic organisms, which are important indicators of ecosystem health.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Marine Studies Publications
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Created: Thu, 12 Feb 2009, 13:48:27 EST by Ms Karen Naughton on behalf of Centre for Marine Studies