Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase

Nicolas, Cécile, Kiefer, Patrick, Letisse, Fabien, Kromer, Jens, Massou, Stéphane, Soucaille, Philippe, Wittmann, Christoph, Lindley, Nic D. and Portais, Jean-Charles (2007) Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase. FEBS Letters, 581 20: 3771-3776. doi:10.1016/j.febslet.2007.06.066


Author Nicolas, Cécile
Kiefer, Patrick
Letisse, Fabien
Kromer, Jens
Massou, Stéphane
Soucaille, Philippe
Wittmann, Christoph
Lindley, Nic D.
Portais, Jean-Charles
Title Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase
Formatted title
Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
1873-3468
Publication date 2007-08-07
Sub-type Article (original research)
DOI 10.1016/j.febslet.2007.06.066
Volume 581
Issue 20
Start page 3771
End page 3776
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Subject 060101 Analytical Biochemistry
060114 Systems Biology
Abstract The deletion of the zwf gene encoding G6PDH activity led to restructuring of the carbon flux through central metabolism in Escherichia coli, though over-expression of this gene had only minor consequences for overall carbon flux. The modified carbon flux seen in the zwf deletion mutant enabled alternative routes of anabolic precursor formation and an adequate supply of NADPH synthesis via a modified TCA cycle to be generated so as to sustain growth rates comparable to the WT.
Keyword Metabolic network
Fluxomics
C-labelling experiments
Mass isotopomer analysis
Escherichia coli
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 12 Feb 2009, 20:30:06 EST by Gina Velli on behalf of Aust Institute for Bioengineering & Nanotechnology