Activation of the MAPK pathway is a common event in uveal melanomas although it rarely occurs through mutation of BRAF or RAS

Zuidervaart, W., van Nieuwpoort, F., Stark, M., Dijkman, R., Packer, L., Borgstein, A. -M., Pavey, S., van der Velden, P., Out, C., Jager, M. J., Hayward, N. K. and Gruis, N. A. (2005) Activation of the MAPK pathway is a common event in uveal melanomas although it rarely occurs through mutation of BRAF or RAS. British Journal of Cancer, 92 11: 2032-2038. doi:10.1038/sj.bjc.6602598


Author Zuidervaart, W.
van Nieuwpoort, F.
Stark, M.
Dijkman, R.
Packer, L.
Borgstein, A. -M.
Pavey, S.
van der Velden, P.
Out, C.
Jager, M. J.
Hayward, N. K.
Gruis, N. A.
Title Activation of the MAPK pathway is a common event in uveal melanomas although it rarely occurs through mutation of BRAF or RAS
Formatted title
Activation of the MAPK pathway is a common event in uveal melanomas although it rarely occurs through mutation of BRAF or RAS
Journal name British Journal of Cancer   Check publisher's open access policy
ISSN 0007-0920
1532-1827
Publication date 2005-06-01
Sub-type Article (original research)
DOI 10.1038/sj.bjc.6602598
Open Access Status DOI
Volume 92
Issue 11
Start page 2032
End page 2038
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract In contrast to cutaneous melanoma, there is no evidence that BRAF mutations are involved in the activation of the mitogen-activated protein kinase (MAPK) pathway in uveal melanoma, although there is increasing evidence that this pathway is activated frequently in the latter tumours. In this study, we performed mutation analysis of the RAS and BRAF genes in a panel of 11 uveal melanoma cell lines and 19 primary uveal melanoma tumours. In addition, Western blot and immunohistochemical analyses were performed on downstream members of the MAPK pathway in order to assess the contribution of each of these components. No mutations were found in any of the three RAS gene family members and only one cell line carried a BRAF mutation (V599E). Despite this, mitogenactivated protein kinase/extracellular signal-regulated kinase kinase (MEK), ERK and ELK were constitutively activated in all samples. These data suggest that activation of the MAPK pathway is commonly involved in the development of uveal melanoma, but occurs through a mechanism different to that of cutaneous melanoma.
Keyword MAPK pathway
Uveal melanoma
BRAF
RAS
Mutation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Thu, 12 Feb 2009, 02:26:37 EST by Alexandra Cooney on behalf of UQ Diamantina Institute