Domain Topology of the p62 Complex Within the 3-D Architecture of the Nuclear Pore Complex

Schwarz-Herion, Kyrill, Maco, Bohumil, Sauder, Ursula and Fahrenkrog, Birthe (2007) Domain Topology of the p62 Complex Within the 3-D Architecture of the Nuclear Pore Complex. Journal of Molecular Biology, 370 4: 796-806. doi:10.1016/j.jmb.2007.05.030


Author Schwarz-Herion, Kyrill
Maco, Bohumil
Sauder, Ursula
Fahrenkrog, Birthe
Title Domain Topology of the p62 Complex Within the 3-D Architecture of the Nuclear Pore Complex
Journal name Journal of Molecular Biology   Check publisher's open access policy
ISSN 0022-2836
Publication date 2007-07-20
Sub-type Article (original research)
DOI 10.1016/j.jmb.2007.05.030
Volume 370
Issue 4
Start page 796
End page 806
Total pages 11
Place of publication Oxford
Publisher Elsevier
Language eng
Subject 0601 Biochemistry and Cell Biology
Abstract The nuclear pore complex (NPC) is the only known gateway for exchange of macromolecules between the cytoplasm and nucleus of eukaryotic cells. One key compound of the NPC is the p62 subcomplex, which consists of the nucleoporins p62, p54, and p58/p45 and is supposed to be involved in nuclear protein import and export. Here we show the localization of distinct domains of the p62 complex by immuno-electron microscopy using isolated nuclei from Xenopus oocytes. To determine the exact position of the p62 complex, we examined the localization of the C and N-terminal domains of p62 by immunogold-labeling using domain-specific antibodies against p62. In addition we expressed epitope-tagged versions of p62, p54, and p58 in Xenopus oocytes and localized the domains with antibodies against the tags. This first systematic analysis of the domain topology of the p62 complex within the NPC revealed that the p62 complex is anchored to the cytoplasmic face of the NPC most likely by the coiled-coil domains of the three nucleoporins. Furthermore, we found the phenylalanine-glycine (FG)-repeat domain of p62, but not of p58 and p54, to be of mobile and flexible nature.
Keyword nuclear pore complex
p62 complex
nucleoporins
FG-repeats
immuno-electron microscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 11 Feb 2009, 09:53:57 EST by Maryanne Watson on behalf of Faculty of Science