Hyperoxia inhibits several critical aspects of vascular development

Uno, Koichi, Merges, Carol A., Grebe, Rhonda, Lutty, Gerard A. and Prow, Tarl W. (2007) Hyperoxia inhibits several critical aspects of vascular development. Developmental Dynamics, 236 4: 981-990. doi:10.1002/dvdy.21122

Author Uno, Koichi
Merges, Carol A.
Grebe, Rhonda
Lutty, Gerard A.
Prow, Tarl W.
Title Hyperoxia inhibits several critical aspects of vascular development
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1097-0177
Publication date 2007-04
Sub-type Article (original research)
DOI 10.1002/dvdy.21122
Volume 236
Issue 4
Start page 981
End page 990
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Subject 060103 Cell Development, Proliferation and Death
Abstract Normal human retinal vascular development uses angiogenesis and vasculogenesis, both of which are interrupted in the vaso-obliteration phase of retinopathy of prematurity (ROP). Canine oxygen-induced retinopathy (OIR) closely resembles human ROP. Canine retinal endothelial cells (ECs) and angioblasts were used to model OIR and characterize the effects of hyperoxia on angiogenesis and vasculogenesis. Cell cycle analysis showed that hyperoxia reduced the number of G1 phase cells and showed increased arrest in S phase for both cell types. Migration of ECs was significantly inhibited in hyperoxia (P < 0.01). Hyperoxia disrupted the cytoskeleton of angioblasts but not ECs after 2 days. Differentiation of angioblasts into ECs (determined by acetylated low-density lipoprotein uptake) was evaluated after basic fibroblast growth factor treatment. Differentiation of angioblasts into pericytes was determined by smooth muscle actin expression after treatment with platelet-derived growth factor. Differentiation into ECs was significantly inhibited by hyperoxia (P < 0.0001). The percentage of CXCR4+ cells (a marker for retinal vascular precursors) increased in both treatment groups after hyperoxia. These data show novel mechanisms of hyperoxia-induced disruption of vascular development.
Keyword Angioblasts
Endothelial cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Tue, 10 Feb 2009, 16:06:20 EST by Laura McTaggart on behalf of Aust Institute for Bioengineering & Nanotechnology