Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines

McMeniman, Conor J., Lane, Amanda M., Fong, Amy W. C., Voronin, Denis A., Iturbe-Ormaetxe, Inaki, Yamada, Ryuichi, McGraw, Elizabeth A. and O'Neill, Scott L. (2008) Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines. Applied and Environmental Microbiology, 74 22: 6963-6969. doi:10.1128/AEM.01038-08

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Author McMeniman, Conor J.
Lane, Amanda M.
Fong, Amy W. C.
Voronin, Denis A.
Iturbe-Ormaetxe, Inaki
Yamada, Ryuichi
McGraw, Elizabeth A.
O'Neill, Scott L.
Title Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
1098-5336
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1128/AEM.01038-08
Open Access Status File (Publisher version)
Volume 74
Issue 22
Start page 6963
End page 6969
Total pages 7
Place of publication Washington, D.C., U.S.A.
Publisher American Society for Microbiology
Collection year 2009
Language eng
Subject C1
960499 Control of Pests, Diseases and Exotic Species not elsewhere classified
060307 Host-Parasite Interactions
060303 Biological Adaptation
060599 Microbiology not elsewhere classified
Abstract The horizontal transfer of the bacterium Wolbachia pipientis between invertebrate hosts hinges on the ability of Wolbachia to adapt to new intracellular environments. The experimental transfer of Wolbachia between distantly related host species often results in the loss of infection, presumably due to an inability of Wolbachia to adapt quickly to the new host. To examine the process of adaptation to a novel host, we transferred a life-shortening Wolbachia strain, wMelPop, from the fruit fly Drosophila melanogaster into a cell line derived from the mosquito Aedes albopictus. After long-term serial passage in this cell line, we transferred the mosquito-adapted wMelPop into cell lines derived from two other mosquito species, Aedes aegypti and Anopheles gambiae. After a prolonged period of serial passage in mosquito cell lines, wMelPop was reintroduced into its native host, D. melanogaster, by embryonic microinjection. The cell line-adapted wMelPop strains were characterized by a loss of infectivity when reintroduced into the original host, grew to decreased densities, and had reduced abilities to cause life-shortening infection and cytoplasmic incompatibility compared to the original strain. We interpret these shifts in phenotype as evidence for genetic adaptation to the mosquito intracellular environment. The use of cell lines to preadapt Wolbachia to novel hosts is suggested as a possible strategy to improve the success of transinfection in novel target insect species. Copyright © 2008, American Society for Microbiology
Keyword Wolbachia pipientis
Aedes albopictus
Novel hosts
Drosophila melanogaster
Adaptation
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 09 Feb 2009, 18:04:15 EST by Gail Walter on behalf of School of Biological Sciences