Protein semi-synthesis: new proteins for functional and structural studies

Durek, Thomas and Becker, Christian F. W. (2005) Protein semi-synthesis: new proteins for functional and structural studies. Biomolecular Engineering, 22 5-6: 153-172. doi:10.1016/j.bioeng.2005.07.004

Author Durek, Thomas
Becker, Christian F. W.
Title Protein semi-synthesis: new proteins for functional and structural studies
Journal name Biomolecular Engineering   Check publisher's open access policy
ISSN 1389-0344
Publication date 2005
Sub-type Article (original research)
DOI 10.1016/j.bioeng.2005.07.004
Volume 22
Issue 5-6
Start page 153
End page 172
Total pages 18
Place of publication United States
Publisher Elsevier
Language eng
Subject 030406 Proteins and Peptides
Abstract Our ability to alter and control the structure and function of biomolecules, and of proteins in particular, will be of utmost importance in order to understand their respective biological roles in complex systems such as living organisms. This challenge has prompted the development of powerful modern techniques in the fields of molecular biology, physical biochemistry and chemical biology. These fields complement each other and their successful combination has provided unique insights into protein structure and function at the level of isolated molecules, cells and organisms. Chemistry is without doubt most suited for introducing subtle changes into biomolecules down to the atomic level, but often struggles when it comes to large targets, such as proteins. In this review, we attempt to give an overview of modern and broadly applicable techniques that permit chemical synthesis to be applied to complex protein targets in order to gain control over their structure and function. As will be demonstrated, these approaches offer unique possibilities in our efforts to understand the molecular basis of protein functioning in vitro and in vivo. We will discuss modern synthetic reactions that can be applied to proteins and give examples of recent highlights. Another focus of this review will be the application of inteins as versatile protein engineering tools.
Keyword Expressed protein ligation
Native chemical ligation
Chemoselective ligations
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 09 Feb 2009, 17:05:42 EST by Gina Velli on behalf of Institute for Molecular Bioscience