Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli

Holden, Nicola J., Totsika, Makrina, Mahler, Eva, Roe, Andrew J., Catherwood, Kirsteen, Lindner, Karin, Dobrindt, Ulrich and Gally, David L. (2006) Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli. Microbiology, 152 4: 1143-1153. doi:10.1099/mic.0.28677-0


Author Holden, Nicola J.
Totsika, Makrina
Mahler, Eva
Roe, Andrew J.
Catherwood, Kirsteen
Lindner, Karin
Dobrindt, Ulrich
Gally, David L.
Title Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli
Formatted title
Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli
Journal name Microbiology   Check publisher's open access policy
ISSN 1350-0872
1465-2080
Publication date 2006-04
Sub-type Article (original research)
DOI 10.1099/mic.0.28677-0
Volume 152
Issue 4
Start page 1143
End page 1153
Total pages 11
Place of publication Reading, England
Publisher Society for General Microbiology
Language eng
Subject 0605 Microbiology
Formatted abstract
The majority of Escherichia coli strains isolated from urinary tract infections have the potential to express multiple fimbriae. Two of the most common fimbrial adhesins are type 1 fimbriae and pyelonephritis-associated pili (Pap). Previous research has shown that induced, plasmid-based expression of a Pap regulator, papB, and its close homologues can prevent inversion of the fim switch controlling the expression of type 1 fimbriae. The aim of the present study was to determine if this cross-regulation occurs when PapB is expressed from its native promoter in the chromosome of E. coli K-12 and clinical isolates. The regulation was examined in three ways: (1) mutated alleles of the pap regulatory region, including papB and papI, that maintain the pap promoter in either the off or the on phase were exchanged into the chromosome of both E. coli K-12 and the clinical isolate E. coli CFT073, and the effect on type 1 fimbrial expression was measured; (2) type 1 fimbrial expression was determined using a novel fimS : : gfp+ reporter system in mutants of the clinical isolate E. coli 536 in which combinations of complete fimbrial clusters had been deleted; (3) type 1 fimbrial expression was determined in a range of clinical isolates and compared with both the number of P clusters and their expression. All three approaches demonstrated that P expression represses type 1 fimbrial expression. Using a number of novel genetic approaches, this work extends the initial finding that PapB inhibits FimB recombination to the impact of this regulation in clinical isolates.
Keyword Microbiology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 06 Feb 2009, 15:24:16 EST by Ms Karen Naughton on behalf of School of Chemistry & Molecular Biosciences