Caveolin-1 polarization in migrating endothelial cells is directed by substrate topology not chemoattractant gradient

Santilman, Virginie, Baran, JoAnne, Anand-Apte, Bela, Fox, Paul L. and Parat, Marie-Odile (2006) Caveolin-1 polarization in migrating endothelial cells is directed by substrate topology not chemoattractant gradient. Cell Motility and the Cytoskeleton, 63 11: 673-680.


Author Santilman, Virginie
Baran, JoAnne
Anand-Apte, Bela
Fox, Paul L.
Parat, Marie-Odile
Title Caveolin-1 polarization in migrating endothelial cells is directed by substrate topology not chemoattractant gradient
Journal name Cell Motility and the Cytoskeleton   Check publisher's open access policy
ISSN 1097-0169
0886-1544
Publication date 2006-11
Sub-type Article (original research)
DOI 10.1002/cm.20153
Volume 63
Issue 11
Start page 673
End page 680
Total pages 8
Place of publication Malden, USA
Publisher Wiley Interscience
Language eng
Subject 0601 Biochemistry and Cell Biology
Abstract Polarization is a hallmark of migrating cells, and an asymmetric distribution of proteins is essential to the migration process. Caveolin-1 is highly polarized in migrating endothelial cells (EC). Several studies have shown caveolin-1 accumulation in the front of migrating EC while others report its accumulation in the EC rear. In this paper we address these conflicting results on polarized localization of caveolin-1. We find evidence for the hypothesis that different modes of locomotion lead to differences in protein polarization. In particular, we show that caveolin-1 is primarily localized in the rear of cells migrating on a planar substrate, but in the front of cells traversing a three-dimensional pore. We also show that a chemoattractant, present either as a gradient or ubiquitously in the medium, does not alter caveolin-1 localization in cells in either mode of locomotion. Thus we conclude that substrate topology, and not the presence of a chemoattractant, directs the polarization of caveolin-1 in motile ECs.
Keyword chemotaxis
chemokinesis
cell migration
motility
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Pharmacy Publications
 
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