Differential Caveolin-1 Polarization in Endothelial Cells during Migration in Two and Three Dimensions

Parat, M. O., Anande-Apte, B. and Fox, P. L. (2003) Differential Caveolin-1 Polarization in Endothelial Cells during Migration in Two and Three Dimensions. Molecular biology of the cell, 14 8: 3156-3168. doi:10.1091/mbc.E02-11-0761

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Author Parat, M. O.
Anande-Apte, B.
Fox, P. L.
Title Differential Caveolin-1 Polarization in Endothelial Cells during Migration in Two and Three Dimensions
Journal name Molecular biology of the cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2003-08
Sub-type Article (original research)
DOI 10.1091/mbc.E02-11-0761
Open Access Status File (Publisher version)
Volume 14
Issue 8
Start page 3156
End page 3168
Total pages 13
Editor Anthony Bretscher
Place of publication Bethesda, MD
Publisher American Society for Cell Biology
Language eng
Subject 0601 Biochemistry and Cell Biology
Abstract Endothelial cell (EC) migration is a critical event during multiple physiological and pathological processes. ECs move in the plane of the endothelium to heal superficially injured blood vessels but migrate in three dimensions during angiogenesis. We herein investigate differences in these modes of movement focusing on caveolae and their defining protein caveolin-1. Using a novel approach for morphological analysis of transmigrating cells, we show that ECs exhibit a polarized distribution of caveolin-1 when traversing a filter pore. Strikingly, in these cells caveolin-1 seems to be released from caveolar structures in the cell rear and to relocalize at the cell front in a cytoplasmic form. In contrast, during planar movement caveolin-1 is concentrated at the rear of ECs, colocalizing with caveolae. The phosphorylatable Tyr14 residue of caveolin-1 is required for polarization of the protein during transmigration but does not alter polarization during planar movement. Palmitoylation of caveolin-1 is not essential for redistribution of the protein during either mode of movement. Thus, ECs migrating in three dimensions uniquely exhibit dissociation of caveolin-1 from caveolae and phosphorylation-dependent relocalization to the cell front.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Pharmacy Publications
 
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