Gravity spun polycaprolactone fibres for soft tissue engineering: Interaction with fibroblasts and myoblasts in cell culture

Williamson, Matthew Richard, Adams, Eric F. and Coombes, Allan G. A. (2006) Gravity spun polycaprolactone fibres for soft tissue engineering: Interaction with fibroblasts and myoblasts in cell culture. Biomaterials, 27 7: 1019-1026. doi:10.1016/j.biomaterials.2005.06.018


Author Williamson, Matthew Richard
Adams, Eric F.
Coombes, Allan G. A.
Title Gravity spun polycaprolactone fibres for soft tissue engineering: Interaction with fibroblasts and myoblasts in cell culture
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2006-03
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2005.06.018
Volume 27
Issue 7
Start page 1019
End page 1026
Total pages 8
Place of publication United Kingdom
Publisher Elsevier
Language eng
Subject 111504 Pharmaceutical Sciences
Abstract Poly(ε-caprolactone) (PCL) fibres were produced by wet spinning from solutions in acetone under low shear (gravity flow) conditions. As-spun PCL fibres exhibited a mean strength and stiffness of 7.9 MPa and 0.1 GPa, respectively and a rough, porous surface morphology. Cold drawing to an extension of 500% resulted in increases in fibre strength (43 MPa) and stiffness (0.3 GPa) and development of an oriented, fibrillar surface texture. The proliferation rate of Swiss 3T3 mouse fibroblasts and C2C12 mouse myoblasts on as-spun, 500% cold-drawn and gelatin-modified PCL fibres was determined in cell culture to provide a basic measure of the biocompatibility of the fibres. Proliferation of both cell types was consistently higher on gelatin-coated fibres relative to as-spun fibres at time points below 7 days. Fibroblast growth rates on cold-drawn PCL fibres exceeded those on as-spun fibres but myoblast proliferation was similar on both substrates. After 1 day in culture, both cell types had spread and coalesced on the fibres to form a cell layer, which conformed closely to the underlying topography. The high fibre compliance combined with a potential for modifying the fibre surface chemistry with cell adhesion molecules and the surface architecture by cold drawing to enhance proliferation of fibroblasts and myoblasts, recommends further investigation of gravity-spun PCL fibres for 3-D scaffold production in soft tissue engineering.
Keyword Biomedical materials
Polycaprolactone
Fibres
Tissue engineering
Fibroblasts
Myoblasts
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Pharmacy Publications
 
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Created: Wed, 04 Feb 2009, 15:54:59 EST by Ms Karen Naughton on behalf of School of Pharmacy