Purification of transferrin from Cohn supernatant I using ion-exchange chromatography

McCann, K. B., Hughes, B., Wu, J., Bertolini, J. and Gomme, P. T. (2005) Purification of transferrin from Cohn supernatant I using ion-exchange chromatography. Biotechnology And Applied Biochemistry, 42 3: 211-217. doi:10.1042/BA20050065


Author McCann, K. B.
Hughes, B.
Wu, J.
Bertolini, J.
Gomme, P. T.
Title Purification of transferrin from Cohn supernatant I using ion-exchange chromatography
Journal name Biotechnology And Applied Biochemistry   Check publisher's open access policy
ISSN 0885-4513
1470-8744
Publication date 2005-05
Sub-type Article (original research)
DOI 10.1042/BA20050065
Volume 42
Issue 3
Start page 211
End page 217
Total pages 7
Place of publication London
Publisher Portland Press
Language eng
Subject 100302 Bioprocessing, Bioproduction and Bioproducts
Abstract The present paper describes an anion-exchange chromatography method to separate iron-free apo-Tf (apo-transferrin) from albumin and IgG in Cohn supernatant I. The method uses DEAE-fast flow Sepharose chromatography along with optimized protein concentration (5%, w/v) and column operation conditions (40 g/l, conductivity <1.0 mS/cm) to resolve apo-Tf from IgG and albumin. The single step purifies apo-Tf to >90% and provides an efficient means to obtain commercial quantities of the protein. doi:
Keyword Cohn supernatant I
human serum albumin (HSA)
ion-exchange chromatography
iron transport
plasma
transferrin
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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