Regulation of P-fimbrial phase variation frequencies in Escherichia coli CFT073

Holden, Nicola, Totsika, Makrina, Dixon, Lynn, Catherwood, Kirsteen and Gally, David L. (2007) Regulation of P-fimbrial phase variation frequencies in Escherichia coli CFT073. Infection and Immunity, 75 7: 3325-3334. doi:10.1128/IAI.01989-06

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Author Holden, Nicola
Totsika, Makrina
Dixon, Lynn
Catherwood, Kirsteen
Gally, David L.
Title Regulation of P-fimbrial phase variation frequencies in Escherichia coli CFT073
Formatted title
Regulation of P-fimbrial phase variation frequencies in Escherichia coli CFT073
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2007-07
Sub-type Article (original research)
DOI 10.1128/IAI.01989-06
Open Access Status File (Publisher version)
Volume 75
Issue 7
Start page 3325
End page 3334
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Adherence of uropathogenic Escherichia coli to host tissue is required for infection and is mediated by fimbriae, such as pyelonephritis-associated pili (Pap). Expression of P fimbriae is regulated by phase variation, and to date, phase transition frequencies have been measured only for pap regulatory region constructs integrated into the E. coli K-12 chromosome. The aim of this work was to measure P phase transition frequencies in clinical isolates for the first time, including frequencies for the sequenced strain E. coli CFT073. P fimbriation and associated phase transition frequencies were measured for two E. coli clinical isolates and compared with levels for homologous pap constructs in E. coli K-12. Fimbriation and off-to-on transition frequencies were always higher in the clinical isolate. It was concluded that the regulatory inputs controlling papI expression are likely to be different in E. coli CFT073 and E. coli K-12 as (i) phase variation could be stimulated in E. coli K-12 by induction of papI and (ii) the level of expression of a papI::gfp+ fusion was higher in E. coli CFT073 than in E. coli K-12. Furthermore, phase transition frequencies for the two E. coli CFT073 pap clusters were shown to be different depending on the culture conditions, indicating that there is a hierarchy of expression depending on signal inputs.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Faculty of Science Publications
Excellence in Research Australia (ERA) - Collection
 
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