Microbial community structure of sandy intertidal sediments in the North Sea, Sylt-Rømø Basin, Wadden Sea

Musat, Niculina, Werner, Ursula, Knittel, Katrin, Kolb, Steffen, Dodenhof, Tanja, van Beusekom, Justus E.E., de Beer, Dirk, Dubilier, Nicole and Amann, Rudolf (2006) Microbial community structure of sandy intertidal sediments in the North Sea, Sylt-Rømø Basin, Wadden Sea. Systematic And Applied Microbiology: A Journal of Microbial Diversity, 29 4: 333-348. doi:10.1016/j.syapm.2005.12.006

Author Musat, Niculina
Werner, Ursula
Knittel, Katrin
Kolb, Steffen
Dodenhof, Tanja
van Beusekom, Justus E.E.
de Beer, Dirk
Dubilier, Nicole
Amann, Rudolf
Title Microbial community structure of sandy intertidal sediments in the North Sea, Sylt-Rømø Basin, Wadden Sea
Journal name Systematic And Applied Microbiology: A Journal of Microbial Diversity   Check publisher's open access policy
ISSN 0723-2020
Publication date 2006-06
Sub-type Article (original research)
DOI 10.1016/j.syapm.2005.12.006
Volume 29
Issue 4
Start page 333
End page 348
Total pages 16
Place of publication Oldendorf/Luhe, West Germany
Publisher Inter-Research
Language eng
Subject 060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)
060504 Microbial Ecology
Abstract Molecular biological methods were used to investigate the microbial diversity and community structure in intertidal sandy sediments near the island of Sylt (Wadden Sea) at a site which was characterized for transport and mineralization rates in a parallel study (D. de Beer, F. Wenzhöfer, T. Ferdelman, S.E. Boehme, M. Huettel, J.E.E. van Beusekom, M.E. Böttcher, N. Musat, N. Dubilier, Transport and mineralization rates in North Sea sandy intertidal sediments, Sylt-Romo Basin, Wadden Sea, Limnol. Oceanogr. 50 (2005) 113–127). Comparative 16S rRNA sequence analysis revealed a high bacterial diversity. Most sequences retrieved by PCR with a general bacterial primer set were affiliated with Bacteroidetes, Gammaproteobacteria, Deltaproteobacteria and the Pirellula cluster of Planctomycetales. Fluorescence in situ hybridization (FISH) and slot-blot hybridization with group-specific rRNA-targeted oligonucleotide probes were used to characterize the microbial community structure over depth (0–12 cm) and seasons (March, July, October). We found high abundances of bacteria with total cell numbers up to 3×109 cells ml−1 and a clear seasonal variation, with higher values in July and October versus March. The microbial community was dominated by members of the Planctomycetes, the Cytophaga/Flavobacterium group, Gammaproteobacteria, and bacteria of the Desulfosarcina/Desulfococcus group. The high abundance (1.5×107–1.8×108 cells ml−1 accounting for 3–19% of all cells) of presumably aerobic heterotrophic polymer-degrading planctomycetes is in line with the high permeability, deep oxygen penetration, and the high rates of aerobic mineralization of algal biomass measured in the sandy sediments by de Beer et al. (2005). The high and stable abundance of members of the Desulfosarcina/Desulfococcus group, both over depth and season, suggests that these bacteria may play a more important role than previously assumed based on low sulfate reduction rates in parallel cores (de Beer et al., 2005).
Keyword Bacteriology
16S rRNA diversity
Community structure
Slot-blot hybridization
Marine sediment
Desulfosarcina/Desulfococcus group
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
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Created: Tue, 03 Feb 2009, 15:45:22 EST by Ms Karen Naughton on behalf of Advanced Water Management Centre