A critical time window of Sry action in gonadal sex determination in mice

Hiramatsu, Ryuji, Matoba, Shogo, Kanai-Azuma, Masami, Tsunekawa, Naoki, Katoh-Fukui, Yuko, Kurohmaru, Masamichi, Morohashi, Ken-ichirou, Wilhelm, Dagmar, Koopman, Peter and Kanai, Yoshiakira (2009) A critical time window of Sry action in gonadal sex determination in mice. Development, 136 1: 129-138. doi:10.1242/dev.029587

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Author Hiramatsu, Ryuji
Matoba, Shogo
Kanai-Azuma, Masami
Tsunekawa, Naoki
Katoh-Fukui, Yuko
Kurohmaru, Masamichi
Morohashi, Ken-ichirou
Wilhelm, Dagmar
Koopman, Peter
Kanai, Yoshiakira
Title A critical time window of Sry action in gonadal sex determination in mice
Journal name Development   Check publisher's open access policy
ISSN 0950-1991
1477-9129
Publication date 2009
Year available 2008
Sub-type Article (original research)
DOI 10.1242/dev.029587
Open Access Status File (Publisher version)
Volume 136
Issue 1
Start page 129
End page 138
Total pages 9
Place of publication Cambridge, England
Publisher Company of Biologists
Collection year 2009
Language eng
Subject
970106 Expanding Knowledge in the Biological Sciences
C1
270200 Genetics
Abstract In mammals, the Y-linked sex-determining gene Sry cell-autonomously promotes Sertoli cell differentiation from bipotential supporting cell precursors through SRY-box containing gene 9 (Sox9), leading to testis formation. Without Sry action, the supporting cells differentiate into granulosa cells, resulting in ovarian development. However, how Sry acts spatiotemporally to switch supporting cells from the female to the male pathway is poorly understood. We created a novel transgenic mouse line bearing an inducible Sry transgene under the control of the Hsp70.3 promoter. Analysis of these mice demonstrated that the ability of Sry to induce testis development is limited to approximately 11.0-11.25 dpc, corresponding to a time window of only 6 hours after the normal onset of Sry expression in XY gonads. If Sry was activated after 11.3 dpc, Sox9 activation was not maintained, resulting in ovarian development. This time window is delimited by the ability to engage the high-FGF9/low-WNT4 signaling states required for Sertoli cell establishment and cord organization. Our results indicate the overarching importance of Sry action in the initial 6-hour phase for the female-to-male switching of FGF9/WNT4 signaling patterns.
Keyword Sry
Sox9
Fgf9
Wnt4
Sertoli cells
sex differentiation
Mouse
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 03 Feb 2009, 12:02:18 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience