Epithiospecifier protein activity in broccoli: The link between terminal alkenyl glucosinolates and sulphoraphane nitrile

Williams, D. J., Critchley,C., Pun,S, Nottingham,S and O'Hare, T. J. (2008) Epithiospecifier protein activity in broccoli: The link between terminal alkenyl glucosinolates and sulphoraphane nitrile. Phytochemistry, 69 16: 2765-2773. doi:10.1016/j.phytochem.2008.09.018


Author Williams, D. J.
Critchley,C.
Pun,S
Nottingham,S
O'Hare, T. J.
Title Epithiospecifier protein activity in broccoli: The link between terminal alkenyl glucosinolates and sulphoraphane nitrile
Journal name Phytochemistry   Check publisher's open access policy
ISSN 0031-9422
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1016/j.phytochem.2008.09.018
Open Access Status
Volume 69
Issue 16
Start page 2765
End page 2773
Total pages 9
Place of publication United Kingdom
Publisher Pergamon
Collection year 2009
Language eng
Subject C1
929999 Health not elsewhere classified
060199 Biochemistry and Cell Biology not elsewhere classified
Abstract The chemical nature of the hydrolysis products from the glucosinolate-myrosinase system depends on the presence or absence of supplementary proteins, such as epithiospecifier proteins (ESPs). ESPs (noncatalytic cofactors of myrosinase) promote the formation of epithionitriles from terminal alkenyl glucosinolates and as recent evidence suggests, simple nitriles at the expense of isothiocyanates. The ratio of ESP activity to myrosinase activity is crucial in determining the proportion of these nitriles produced on hydrolysis. Sulphoraphane, a major isothiocyanate produced in broccoli seedlings, has been found to be a potent inducer of phase 2 detoxification enzymes. However, ESP may also support the formation of the non-inductive sulphoraphane nitrile. Our objective was to monitor changes in ESP activity during the development of broccoli seedlings and link these activity changes with myrosinase activity, the level of terminal alkenyl glucosinolates and sulphoraphane nitrile formed. Here, for the first time, we show ESP activity increases up to day 2 after germination before decreasing again to seed activity levels at day 5. These activity changes paralleled changes in myrosinase activity and terminal alkenyl glucosinolate content. There is a significant relationship between ESP activity and the formation of sulforaphane nitrile in broccoli seedlings. The significance of these findings for the health benefits conferred by eating broccoli seedlings is briefly discussed. Crown
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Mon, 02 Feb 2009, 16:19:02 EST by Gail Walter on behalf of School of Biological Sciences