Spherical and cylindrical microencapsulation of living cells using microfluidic devices

Hong, Joung Sook, Shin, Su Jung, Lee, SangHoon, Wong, Edeline and Cooper-White, Justin (2007) Spherical and cylindrical microencapsulation of living cells using microfluidic devices. Korea-Australia Rheology Journal, 19 3: 157-164.

Author Hong, Joung Sook
Shin, Su Jung
Lee, SangHoon
Wong, Edeline
Cooper-White, Justin
Title Spherical and cylindrical microencapsulation of living cells using microfluidic devices
Journal name Korea-Australia Rheology Journal   Check publisher's open access policy
ISSN 1226-119X
Publication date 2007-11
Sub-type Article (original research)
Volume 19
Issue 3
Start page 157
End page 164
Total pages 8
Place of publication Seoul, Republic of Korea
Publisher Korean Society of Rheology
Language eng
Subject 091599 Interdisciplinary Engineering not elsewhere classified
Formatted abstract
Microencapsulation of cells within microfluidic devices enables explicit control of the membrane thickness or cell density, resulting in improved viability of the transplanted cells within an aggressive immune system. In this study, living cells (3T3 and L929 fibroblast cells) are encapsulated within a semi-permeable membrane (calcium crosslinked alginate gel) in two different device designs, a flow focusing and a core-annular flow focusing geometry. These two device designs produce a bead and a long microfibre, respectively. For the alginate bead, an alginate aqueous solution incorporating cells flows through a flow focusing channel and an alginate droplet is formed from the balance of interfacial forces and viscous drag forces resulting from the continuous (oil) phase flowing past the alginate solution. It immediately reacts with an adjacent CaCl2 drop that is extruded into the main flow channel by another flow focusing channel downstream of the site of alginate drop creation. Depending on the flow conditions, monodisperse microbeads of sizes ranging from 50-200 μm can be produced. In the case of the microfibre, the alginate solution with cells is extruded into a continuous phase of CaCl2 solution. The diameter of alginate fibres produced via this technique can be tightly controlled by changing both flow rates. Cell viability in both forms of alginate encapsulant was confirmed by a LIVE/DEAD cell assay for periods of up to 24 hours post encapsulation.
Keyword Calcium alginate gel
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Mon, 02 Feb 2009, 09:48:25 EST by Maryanne Watson on behalf of Aust Institute for Bioengineering & Nanotechnology