Detection of toxoplasmic lesions in mouse brain by USPIO-enhanced magnetic resonance imaging

Wei, Li, Zhou, Gang, Li, Zhen, He, Li, Gao, Mingyuan, Tan, Jinquan and Le, Hao (2007) Detection of toxoplasmic lesions in mouse brain by USPIO-enhanced magnetic resonance imaging. Magnetic Resonance Imaging, 25 10: 1442-1448. doi:10.1016/j.mri.2007.04.016


Author Wei, Li
Zhou, Gang
Li, Zhen
He, Li
Gao, Mingyuan
Tan, Jinquan
Le, Hao
Title Detection of toxoplasmic lesions in mouse brain by USPIO-enhanced magnetic resonance imaging
Journal name Magnetic Resonance Imaging   Check publisher's open access policy
ISSN 0730-725X
1873-5894
Publication date 2007-12
Sub-type Article (original research)
DOI 10.1016/j.mri.2007.04.016
Volume 25
Issue 10
Start page 1442
End page 1448
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Subject 030306 Synthesis of Materials
030302 Nanochemistry and Supramolecular Chemistry
030603 Colloid and Surface Chemistry
030605 Solution Chemistry
030701 Quantum Chemistry
Formatted abstract
The objective of this study was to examine the feasibility of detecting toxoplasmic brain lesions in a mouse model of cerebral toxoplasmosis by ultrasmall superparamagnetic particles of iron oxide (USPIO)-enhanced magnetic resonance imaging (MRI). Toxoplasmosis encephalitis was induced in Kunming mice by intracerebral injection of Toxoplasma gondii tachyzoites. T2- and T2*-weighted MRI was performed 1, 3, 4, 5 and 6 days after infection before USPIO injection; immediately after USPIO injection; and 24 h later. A comparison of USPIO enhancement and Gd-DTPA enhancement was made in three toxoplasmic mice 4 days after infection. Hematoxylin and eosin staining and Prussian blue staining were performed to detect inflammatory reactions and presence of iron in and around the toxoplasmic brain lesions. Nonenhanced T2-/T2*-weighted imaging detected few abnormalities in the brain up to 5 days. Most mice developed prominent hydrocephalus at 6 days. Gd-DTPA-enhanced imaging showed prominent enhancement of the cerebral ventricles but revealed only few space-occupying lesions in the parenchyma. USPIO-enhanced T2*-weighted imaging showed improved detection of toxoplasmic brain lesions that were invisible to nonenhanced T2-/T2*-weighted imaging and gadolinium-enhanced imaging. Most of the enhancing lesions showed nodular enhancement immediately after USPIO injection, some of which changed appearance 24 h later, having a ring enhancement at the outer rim. It can be concluded that USPIO enhancement of the toxoplasmic lesions may reflect blood–brain barrier impairment and/or inflammatory reactions associated with these lesions. USPIO-enhanced imaging may be used in combination with gadolinium-enhanced imaging to provide better characterization of toxoplasmic brain lesions and, potentially, improve the differential diagnosis of toxoplasmosis encephalitis.
Keyword Magnetic resonance imaging
Toxoplasmosis encephalitis
USPIO
Mouse
Brain
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemical Engineering Publications
 
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