CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells

Herszfeld, Daniella, Wolvetang, Ernst, Langton-Bunker, Emma, Chung, Tung-Liang, Filipczyk, Adam A,, Houssami, Souheir, Pegah Jamshid, Koh, Karen, Laslett, Andrew L., Anna Michalska, Nguyen, Linh, Reubinoff, Benjamin E., Tellis, Irene, Auerbach, Jonathan M., Ording, Carol J., Looijenga, Leendert H. J. and Pera, Martin F. (2006) CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells. Nature Biotechnology, 24 3: 351-357. doi:10.1038/nbt1197

Author Herszfeld, Daniella
Wolvetang, Ernst
Langton-Bunker, Emma
Chung, Tung-Liang
Filipczyk, Adam A,
Houssami, Souheir
Pegah Jamshid
Koh, Karen
Laslett, Andrew L.
Anna Michalska
Nguyen, Linh
Reubinoff, Benjamin E.
Tellis, Irene
Auerbach, Jonathan M.
Ording, Carol J.
Looijenga, Leendert H. J.
Pera, Martin F.
Title CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells
Journal name Nature Biotechnology   Check publisher's open access policy
ISSN 1087-0156
Publication date 2006-02-26
Sub-type Article (original research)
DOI 10.1038/nbt1197
Volume 24
Issue 3
Start page 351
End page 357
Total pages 7
Place of publication New York, N.Y., U.S.A.
Publisher Nature PubLISHING Group
Language eng
Subject 0601 Biochemistry and Cell Biology
060103 Cell Development, Proliferation and Death
Abstract The application of human embryonic stem (hES) cells in regenerative medicine will require rigorous quality control measures to ensure the safety of hES cell–derived grafts. During propagation in vitro, hES cells can acquire cytogenetic abnormalities1, 2, 3 as well as submicroscopic genetic lesions, such as small amplifications or deletions4. Many of the genetic abnormalities that arise in hES cell cultures are also implicated in human cancer development. The causes of genetic instability of hES cells in culture are poorly understood, and commonly used cytogenetic methods for detection of abnormal cells are capable only of low-throughput analysis on small numbers of cells. The identification of biomarkers of genetic instability in hES cells would greatly facilitate the development of culture methods that preserve genomic integrity. Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 96 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 29 Jan 2009, 10:51:34 EST by Maryanne Watson on behalf of Aust Institute for Bioengineering & Nanotechnology