Itinerary of Hepatitis B Viruses: Delineation of Restriction Points Critical for Infectious Entry

Funk, Anneke, Mhamdi, Mouna, Lin, Li, Will, Hans and Sirma, Hüseyin (2004) Itinerary of Hepatitis B Viruses: Delineation of Restriction Points Critical for Infectious Entry. Journal of virology, 78 15: 8289-8300. doi:10.1128/jvi.78.15.8289-8300.2004

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Author Funk, Anneke
Mhamdi, Mouna
Lin, Li
Will, Hans
Sirma, Hüseyin
Title Itinerary of Hepatitis B Viruses: Delineation of Restriction Points Critical for Infectious Entry
Journal name Journal of virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2004-08-01
Year available 2004
Sub-type Article (original research)
DOI 10.1128/jvi.78.15.8289-8300.2004
Open Access Status File (Publisher version)
Volume 78
Issue 15
Start page 8289
End page 8300
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject 060506 Virology
Abstract Little is known about cellular determinants essential for human hepatitis B virus infection. Using the duck hepatitis B virus as a model, we first established a sensitive binding assay for both virions and subviral particles and subsequently elucidated the characteristics of the early viral entry steps. The infection itinerary was found to initiate with the attachment of viral particles to a low number of binding sites on hepatocytes (about 104 per cell). Virus internalization was fully accomplished in less than 3 h but was then followed by a period of unprecedented length, about 14 h, until completion of nuclear import of the viral genome. Steps subsequent to virus entry depended on both intact microtubules and their dynamic turnover but not on actin cytoskeleton. Notably, cytoplasmic trafficking of viral particles and emergence of nuclear covalently closed circular DNA requires microtubules during entry only at and for specific time periods. Taken together, these data disclose for the first time a series of steps and their kinetics that are essential for the entry of hepatitis B viruses into hepatocytes and are different from those of any other virus reported so far.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 35 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 34 times in Scopus Article | Citations
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Created: Thu, 29 Jan 2009, 09:27:52 EST by Ms Lynette Adams on behalf of Institute for Molecular Bioscience