Experts' roundtable: Real time PCR and microbiology

Beld, M. G. H. M., Mackay, I. M., Sloots, T. P. and et. al. (2007). Experts' roundtable: Real time PCR and microbiology. In Ian M. Mackay (Ed.), Real-Time PCR in Microbiology From diagnosis to characterization (pp. 357-444) Norfolk, United Kingdom: Caister Academic.

Author Beld, M. G. H. M.
Mackay, I. M.
Sloots, T. P.
et. al.
Title of chapter Experts' roundtable: Real time PCR and microbiology
Title of book Real-Time PCR in Microbiology From diagnosis to characterization
Place of Publication Norfolk, United Kingdom
Publisher Caister Academic
Publication Year 2007
Sub-type Research book chapter (original research)
ISBN 9781904455189
Editor Ian M. Mackay
Chapter number 10
Start page 357
End page 444
Total pages 88
Total chapters 10
Language eng
Subjects 1101 Medical Biochemistry and Metabolomics
1103 Clinical Sciences
06 Biological Sciences
Formatted Abstract/Summary
This chapter attempts to document informal discussions about topics of interest to those of us who develop, employ or make decisions based upon the use of real-time PCR in microbiology. It is also intended to complement the preceding chapters. The layout for this chapter was loosely based on the format of published conference discussions and popular magazine interviews. The authors were most generous with their time and very responsive to badgering. The result is a wander through the minds of those whose papers we read, whose work we admire and whose methods we often employ. Please enjoy reading the opinions provided by a diverse selection of the foremost scientists and clinicians actively working in and around the field of microbiology.
Copyright © 2007 Caister Academic Press
Keyword Microbial quantification
Target preparation
Spin column
Hydrolysis oligoprobes
Fluorogenic chemistries
Microbial template
Fluorogenic oligoprobes
Singleplex assays
Hairpin oligoprobes
Fluorescence melting curve analysis
Duplex assay
Amplicon strand
Quasispecies variation
Microbial diagnostics
Amplicon accumulation
Quantitative molecular methods
External calibrators
Bioterror agents
Melting analysis
Hydrolysis probe
Internal amplification control
Scorpion primers
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Author list: M.G.H.M. Beld, C. Birch, P.A. Cane, W. Carman, E.C.J. Claas, J.P. Clewley, E. Domingo, J. Druce, C. Escarmis, R.A.M. Fouchier, V. Foulongne, M.G. Ison, L.C. Jennings, B. Kaltenboeck, I.D. Kay, M. Kubista, O. Landt, I.M. Mackay, J. Mackay, H.G.M. Niesters, M.D. Nissen, S. Palladino, N.G. Papadopoulos, A. Petrich, M,W. Pfaffl, W. Rawlinson, U. Reischl, N.A. Saunders, C. Savolainen-Kopra, O. Schildgen, G.M. Scott, M. Segondy, R. Seibl, T.P. Sloots, Y.-W. Tang, R. Tellier and P.C.Y. Woo

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Created: Fri, 23 Jan 2009, 15:26:50 EST by Maryanne Watson on behalf of Faculty of Science