Three-Dimensional Cell Culture and Tissue Engineering in a T-CUP (Tissue Culture Under Perfusion)

Timmins, Nicholas E., Scherberich, Arnaud, Früh, Jennifer-Annika, Heberer, Michael, Martin, Ivan and Jakob, Marcel (2007) Three-Dimensional Cell Culture and Tissue Engineering in a T-CUP (Tissue Culture Under Perfusion). Tissue Engineering, 13 8: 2021-2028. doi:10.1089/ten.2006.0158


Author Timmins, Nicholas E.
Scherberich, Arnaud
Früh, Jennifer-Annika
Heberer, Michael
Martin, Ivan
Jakob, Marcel
Title Three-Dimensional Cell Culture and Tissue Engineering in a T-CUP (Tissue Culture Under Perfusion)
Journal name Tissue Engineering   Check publisher's open access policy
ISSN 1076-3279
Publication date 2007-08
Sub-type Article (original research)
DOI 10.1089/ten.2006.0158
Volume 13
Issue 8
Start page 2021
End page 2028
Total pages 8
Place of publication Mary Ann Liebert Publishers
Publisher New Rochelle, N. Y., United States
Language eng
Abstract The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds. The developed Tissue Culture Under Perfusion (T-CUP) bioreactor is based on the concept of controlled and confined alternating motion of scaffolds through a cell suspension or culture medium, as opposed to pumping of the fluid through the scaffolds. Via the T-CUP, articular chondrocytes and bone marrow stromal cells could be seeded into porous scaffolds of different compositions and architectures (chronOS™, Hyaff ®-11, and Polyactive™) at high efficiency (greater than 75%), uniformity (cells were well distributed throughout the scaffold pores), and viability (greater than 97%). Culture of articular chondrocytes seeded into 4-mm thick Polyactive™ scaffolds for 2 weeks in the T-CUP resulted in uniform deposition of cartilaginous matrix. Cultivation of freshly isolated human bone marrow nucleated cells seeded into ENGipore ceramic scaffolds for 19 days in the T-CUP resulted in stromal cell-populated constructs capable of inducing ectopic bone formation in nude mice. The T-CUP bioreactor represents an innovative approach to simple, efficient, and reliable 3D cell culture, and could be used either as a model to investigate mechanisms of tissue development or as a graft manufacturing system in the context of regenerative medicine.
Keyword Porous scaffolds
Tissue Culture Under Perfusion (T-CUP)
Chondrocytes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Later Title: Tissue engineering. Part A Tissue engineering. Part B, Reviews Tissue engineering. Part C, Methods

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 22 Jan 2009, 13:03:01 EST by Ms Karen Naughton on behalf of Aust Institute for Bioengineering & Nanotechnology