Uteroplacental restriction in the rat impairs fetal growth in association with alterations in placental growth factors including PTHrP

Wlodek, Mary E., Westcott, Kerryn T., O’Dowd, Rachael, Serruto, Anne, Wassef, Lesley, Moritz, Karen M. and Moseley, Jane M. (2005) Uteroplacental restriction in the rat impairs fetal growth in association with alterations in placental growth factors including PTHrP. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 288 6: R1620-R1627. doi:10.1152/ajpregu.00789.2004


Author Wlodek, Mary E.
Westcott, Kerryn T.
O’Dowd, Rachael
Serruto, Anne
Wassef, Lesley
Moritz, Karen M.
Moseley, Jane M.
Title Uteroplacental restriction in the rat impairs fetal growth in association with alterations in placental growth factors including PTHrP
Journal name American Journal of Physiology - Regulatory, Integrative and Comparative Physiology   Check publisher's open access policy
ISSN 0002-9513
Publication date 2005-06
Sub-type Article (original research)
DOI 10.1152/ajpregu.00789.2004
Volume 288
Issue 6
Start page R1620
End page R1627
Total pages 8
Place of publication United States
Publisher American Physiological Society
Language eng
Subject 111603 Systems Physiology
110201 Cardiology (incl. Cardiovascular Diseases)
110312 Nephrology and Urology
111401 Foetal Development and Medicine
Abstract During pregnancy, parathyroid hormone-related protein (PTHrP) is one of many growth factors that play important roles to promote fetal growth and development, including stimulation of placental calcium transport. Angiotensin II, acting through the AT1a receptor, is also known to promote placental growth. We examined the effects of bilateral uterine artery and vein ligation (restriction), which mimics placental insufficiency in humans, on growth, intrauterine PTHrP, placental AT1a, and pup calcium. Growth restriction was surgically induced on day 18 of pregnancy in Wistar-Kyoto female rats by uterine vessel ligation. Uteroplacental insufficiency reduced fetal body weight by 15% and litter size (P < 0.001) compared with the control rats with no effect on placental weight or amniotic fluid volume. Uteroplacental insufficiency reduced placental PTHrP content by 46%, with increases in PTHrP (by 2.6-fold), parathyroid hormone (PTH)/PTHrP receptor (by 11.6-fold), and AT1a (by 1.7-fold) relative mRNA in placenta following restriction compared with results in control (P < 0.05). There were no alterations in uterine PTHrP and PTH/PTHrP receptor mRNA expression. Maternal and fetal plasma PTHrP and calcium concentrations were unchanged. Although fetal total body calcium was not altered, placental restriction altered perinatal calcium homeostasis, as evidenced by lower pup total body calcium after birth (P < 0.05). The increased uterine and amniotic fluid PTHrP (P < 0.05) may be an attempt to compensate for the induced impaired placental function. The present study demonstrates that uteroplacental insufficiency alters intrauterine PTHrP, placental AT1a expression, and perinatal calcium in association with a reduction in fetal growth. Uteroplacental insufficiency may provide an important model for exploring the early origins of adult diseases. parathyroid hormone-related protein; growth restriction; calcium; placenta
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 46 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 50 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 19 Jan 2009, 10:16:33 EST by Judy Dingwall on behalf of School of Biomedical Sciences