Polyene-lipids: A new tool to image lipids

Kuerschner, Lars, Ejsing, Christer S., Ekroos, Kim, Shevchenko, Andrej, Anderson, Kurt I and Thiele, Christoph (2005) Polyene-lipids: A new tool to image lipids. Nature Methods, 2 1: 39-45. doi:10.1038/NMETH728

Author Kuerschner, Lars
Ejsing, Christer S.
Ekroos, Kim
Shevchenko, Andrej
Anderson, Kurt I
Thiele, Christoph
Title Polyene-lipids: A new tool to image lipids
Journal name Nature Methods   Check publisher's open access policy
ISSN 1548-7091
Publication date 2005-01
Year available 2005
Sub-type Article (original research)
DOI 10.1038/NMETH728
Volume 2
Issue 1
Start page 39
End page 45
Total pages 7
Place of publication New York
Publisher Nature Pub. Group
Collection year 2005
Language eng
Subject 060104 Cell Metabolism
Abstract Microscopy of lipids in living cells is currently hampered by a lack of adequate fluorescent tags. The most frequently used tags, NBD and BODIPY, strongly influence the properties of lipids, yielding analogs with quite different characteristics. Here, we introduce polyene-lipids containing five conjugated double bonds as a new type of lipid tag. Polyene-lipids exhibit a unique structural similarity to natural lipids, which results in minimal effects on the lipid properties. Analyzing membrane phase partitioning, an important biophysical and biological property of lipids, we demonstrated the superiority of polyene-lipids to both NBD- and BODIPY-tagged lipids. Cells readily take up various polyene-lipid precursors and generate the expected end products with no apparent disturbance by the tag. Applying two-photon excitation microscopy, we imaged the distribution of polyene-lipids in living mammalian cells. For the first time, ether lipids, important for the function of the brain, were successfully visualized.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Medline number: 15782159

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 109 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 103 times in Scopus Article | Citations
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